植物逆境相关启动子及功能.pdfVIP

HEREDITAS (Beijing) 2010 3 , 32(3): 229―234 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.00229 植物逆境相关启动子及功能 朱丽萍, 于壮, 邹翠霞, 李秋莉 , 116029 启动子是调控基因表达的重要顺式元件, 在植物基因表达调控过程中起着重要作用。目前植物抗逆基因 工程中, 人们大多使用组成型表达启动子驱动目的基因的表达。组成型表达启动子虽然能提高转基因植株的抗 逆性, 但是其持续过量地表达转化的外源基因会阻碍植物的生长且减少其产量。因此, 只在胁迫条件下才会驱 动外源基因表达的诱导型启动子的研究显得尤其重要, 已成为目前研究的热点。文章综述了受非生物逆境和生 物逆境胁迫诱导的植物基因启动子的种类和功能, 并展望了植物逆境诱导启动子的研究方向和前景。 逆境; 诱导型启动子; 功能分析 Plant stress-inducible promoters and their function ZHU Li-Ping, YU Zhuang, ZOU Cui-Xia, LI Qiu-Li College of Life Sciences, Liaoning Normal University , Dalian 116029, China Abstract: Promoter is an important cis-regulatory element for gene expression and plays an important role in the process of plant gene expression and regulation. Constitutive promoters are being used to drive alien gene expression in most trans- genic engineering. Although constitutive promoters can improve resistance of transgenic plants to abiotic stresses, over- and constitutive-expression of the alien genes have been shown to cause stunted growth and reduction of yield in transgenic plants. Therefore, inducible promoters, which are expressed only when exposed to stresses, are of importance. This paper reviews the types and functions of plant gene promoters induced by bio- and abio-stresses. The prospect of stress-induced promoters was discussed. Keywords: adversity; stress-inducible promoter; function analysis , , , , , , , 5 DNA, , RNA , DNA , , , , 收稿日期: 2009−09−13; 修回日期: 2009−10−28 基金项目: (