眼镜王蛇线粒体基因组全序列分析.pdfVIP

HEREDITAS (Beijing) 2010 7 , 32(7): 719―725 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2010.00719 眼镜王蛇线粒体基因组全序列分析 陈念, 赖小平 , 510006 参照近缘物种线粒体基因序列共设计和合成了8 对引物, 结合Ex Taq-PCR 、TA 克隆和步移测序技术, 文 章首次获得眼镜王蛇线粒体基因组全序列(GenBank 登录号: EU_921899)。该基因组全长17 267 bp, 共编码 13 个蛋白、2 个 rRNA 、23 个 tRNA—— 其中 tRNA-Ile 基因发生了复制, 属于一种新的蛇类物种线粒体基因排列 模式, 另外还含有2 个非编码的富含AT 的控制区。除了8 个tRNA 基因和 1 个蛋白基因由L 链编码外, 其余 均由H 链编码, 其中H 链编码基因的A 和T 含量接近, 而L 链上A 的含量则明显高于T 。基于21 种蛇合并的 “12S ＋16S”rRNA 基因序列的系统发育分析表明, 眼镜王蛇属与眼镜蛇属亲缘关系较近, 两者与环蛇属共同构 成一个单系群。作为国内外眼镜王蛇线粒体基因组全序列的首次报道, 上述结果对于蛇类物种分子系统发育和 进化研究具有重要意义。 眼镜王蛇; 线粒体基因组; 系统发育 Sequencing and analysis of the complete mitochondrial genome of the King Cobra, Ophiophagus hannah (Serpents: Elapidae) CHEN Nian, LAI Xiao-Ping School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China Abstract: We obtained the complete mitochondrial genome of King Cobra(GenBank accession number: EU_921899) by Ex Taq-PCR, TA-cloning and primer-walking methods. This genome is very similar to other vertebrate, which is 17 267 bp in length and encodes 38 genes (including 13 protein-coding, 2 ribosomal RNA and 23 transfer RNA genes) and two long non-coding regions. The duplication of tRNA-Ile gene forms a new mitochondrial gene rearrangement model. Eight tRNA genes and one protein genes were transcribed from L strand, and the other genes were transcribed genes from H strand. Genes on the H strand show a fairly similar content of Adenosine and Thymine respectively, whereas those on the L strand have higher proportion of A than T. Combined rDNA sequence data (12S+16S rRNA) were used to reconstruct the phylog- eny of 21 snake species for which comp