蛙科两栖动物皮肤抗菌肽的分子多样性及功能.pdfVIP

HEREDITAS (Beijing) 2008 10 , 30(10): 1241―1248 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.01241 金莉莉, 王秋雨 , 110036 : 蛙科(Ranidae)是全球分布最广泛的两栖动物, 种类超过 650 种。为开拓和适应广阔的栖息地及多样的生 态环境, 其皮肤腺体中进化产生了结构复杂、种类繁多的抗菌肽。它们除具有广谱抗菌活性外, 还有抗肿瘤、 抗病毒等生物学活性。蛙科动物皮肤抗菌肽起源于共同祖先, 在漫长的进化过程中, 基因发生了多重复制和突 变, 形成了天然抗菌肽的巨大资源库。这些肽在模拟膜的溶剂中几乎都是疏水的, 并带正电荷, 以一种两亲性 的α-螺旋的构象存在。根据氨基酸组成及结构的相似性, 可以将蛙科动物抗菌肽分成 brevinin-1 、esculentin-1 、 esculentin-2 、temporin 、ranalexin 、ranatuerin-1 、ranatuerin-2 、plaustrin 、brevinin-2 、tigerinin 、japonicin 、nigrocin 和 melittin 相关肽等若干个家族。文章结合作者的研究工作, 综述了目前已经鉴定的蛙科动物皮肤抗菌肽的分子多 样性特点、家族性质和生物学活性的研究进展, 并阐述了东北林蛙新家族抗菌肽的特点。 : 蛙科; 抗菌肽家族; 分子多样性; 生物学活性 Molecular diversities and functions of antibacterial peptides from the skins of Ranidae of amphibians JIN Li-Li, WANG Qiu-Yu School of Life Science, Liaoning University, Shenyang 110036, China Abstract: Granular glands in the frog skins of Ranidae of amphibians, a widely distributed group with over 650 spe- cies, synthesize and secrete a remarkably diverse array of peptides with the broad-spectrum antibacterial, antifungal and other biologic activities to protect the organism against a wide range of pathogens, which are believed to have arisen as a result of multiple gene duplication events. Almost without exception, these components are hydrophobic, cationic and form an amphipathic α-helix in a membrane-mimetic solvent. The peptides can be grouped into families on the basis of structural similarity. To date, brevinin-1, esculentin-1, esculentin-2, and temporin peptides, ranalexin, ranatuerin-1, ranatuerin-2 and palustrin, brevinin-2, tigerinin, japonicin, nigrocin and melittin-related peptides have