粟酒裂殖酵母全基因组中含信号肽的蛋白质的研究.pdfVIP

HEREDITAS (Beijing) 2007 2 , 29(2): 250―256 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0250 刘玉岭, 柳云帆, 谢建平 , 400715 : 对粟酒裂殖酵母全基因组3 条染色体上的4,997 个蛋白序列进行了全局性的分析,利用 signalP3.0 软件分 析这些蛋白的N- 末端信号肽序列, 预测有N- 末端分泌信号肽序列的蛋白 196 个;利用TMpred 软件分析跨膜结 构, 预测跨膜蛋白117 个; 使用PrositeScan 程序分析膜脂蛋白的脂结合位点, 预测有膜脂结合蛋白13 个, 进而 预测分泌性蛋白序列66 个。使用Target P 分析66 个分泌蛋白的蛋白序列, 研究这些蛋白在细胞中的定位。这 些分泌蛋白的功能涉及粟酒裂殖酵母的营养、生殖、细胞间以及细胞与环境间的交流等许多方面, 对细胞的生 存和繁殖有重要意义, 在系统生物学的研究中有重要参考价值。粟酒裂殖酵母分泌组的研究也将为粟酒裂殖酵 母作为药物筛选模型以及开发为外源蛋白表达的宿主提供基础。 : 粟酒裂殖酵母; 信号肽; 膜脂蛋白; 膜整合蛋白; 分泌蛋白 Characterization of Schizosaccharomyces pombe secreted proteins LIU Yu-Ling, LIU Yun-Fan, XIE Jian-Ping Institute of Modern Biopharmaceuticals , School of Life Sciences, Southwest University, Chongqing 400715, China Abstract: Secreted proteins play a vital physiological role. Schizosaccharomyces pombe is an important model organism for cell cycle study and a potential useful drug screen model. Secreted proteins also initiate the mating. However, few global studies concerning the secreted proteins of S. pombe was reported. To address this issue, bioinformatics were used to reveal the global secreted proteins of S. pombe. The 4,997 proteins deduced from the S. pombe genome were analyzed by com- bined several programs. One hundred and sixty proteins were identified carrying an NH2-terminal secretory signal peptide by signalP3.0. Among them, 117 proteins are integral membrane proteins(TMpred), 13 proteins are lipoproteins (PrositeS- can), and 66 proteins are secreted proteins. The location of the secreted proteins was also predicted by Target P. Some of the secreted proteins are involved in the nutrition, reproduction, as well as the communication between ce