应用重组自交系群体定位大豆抗虫QTL.pdfVIP

HEREDITAS (Beijing) 2007 9 29(9): 1139 ―1143 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1139 QTL 付三雄, 王慧, 吴娟娟, 刘华, 盖钧镒, 喻德跃 , , , 210095 : 以大豆组合科丰 1 号×南农 1138-2 衍生的重组自交系(RIL)群体为材料构建遗传连锁图谱, 利用软件 Cartographer V.2.5 采用复合区间作图法检测定位大豆抗虫QTL 。以斜纹夜蛾幼虫重为抗性指标, 检测到 1 个 与抗虫性有关的 QTL, 位于G20-O 连锁群上, 其端距离为31.91 cM, 加性效应估计值为0.0408, 对性状变异的 解释率为 11.74 ％; 以蛹重为抗性指标, 检测到 2 个与抗虫性有关的 QTL, 分别位于 G8-D1b+W 和G17-L 连 锁群上, 其端距离分别为 14.71 cM 和0.01 cM, 加性效应估计值分别为−0.0139 和0.0103, 对性状变异的解释率 分别为 11.30 ％和6.36 ％。 : 大豆; 抗虫; 重组自交系(RIL); QTL 定位 Mapping insect resistance QTLs of soybean with RIL population FU San-Xiong, WANG Hui, WU Juan-Juan, LIU Hua, GAI Jun-Yi, YU De-Yue Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China Abstract: A recombinant inbred line (RIL) population, NJRIKY, which was derived from the cross Kefeng 1 ×Nannong 1138-2, was used to constructed the genetic linkage map. Larval weight and pupae weight of cotton worm [Prodenia litura (L.) Fabricius] were examined and used as indicators of resistance. Based on the linkage map con- structed with SSR markers of this RIL population, one QTL responsible for larval weight was mapped on linkage group G20-O and the position was 31.91 cM. The QTL’s additive effect was 0.0408 and explained 11.74 of the total variation of the larval weight. Two QTLs associated with pupae weight were mapped on linkage group G8-D1b+W and G17-L and the positions were 14.71 cM and 0.01 cM,