中华稻蝗不同地理种群遗传多样性的AFLP分析.pdfVIP

HEREDITAS (Beijing) 2010 2 , 32(2): 163―169 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2010.00163 中华稻蝗不同地理种群遗传多样性的AFLP 分析 1 1 1 1 1 2 马晋 , 李涛 , 龙文敏 , 安玮玮 , 郭亚平 , 马恩波 1. , 030006; 2. , 030006 为研究中华稻蝗种群遗传多样性和遗传结构, 文章对我国7 省市的7 个中华稻蝗种群进行AFLP 分析。 选取7 对引物扩增 128 个个体, 共产生336 条带, 多态性条带292 条, 占86.90%。结果表明: 中华稻蝗种群具 有较高的遗传多样性水平, 其中海南万宁种群的遗传多样性高于其他各种群。Mantel 检验(r=0.27, P=0.89)表明 中华稻蝗各种群遗传距离与地理距离间没有显著相关性。种群间具有明显的遗传分化现象。 UPGMA(Unweighted pair group method average) 聚类分析显示, 7 个中华稻蝗种群按地理距离分为3 支: 北方北 京昌平、山西太原和山东济宁为一支; 南方陕西汉中、湖南长沙和广西来宾为一支; 海南万宁单独为一支。上 述结果以及PCA(Principal component analysis)分析均表明由于地理隔离中华稻蝗种群显示出明显的南北分化和 岛屿大陆种群遗传分化现象。 中华稻蝗; AFLP; 遗传多样性; 地理隔离 Genetic diversity of different geographical populations of Oxya chinensis based on AFLP analysis 1 1 1 1 1 2 MA Jin , LI Tao , LONG Wen-Min , AN Wei-Wei , GUO Ya-Ping , MA En-Bo 1. School of Life Science and Technology, Shanxi University, Taiyuan 030006, China; 2. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China Abstract: In order to study the genetic diversity and genetic structure of populations, 7 populations of Oxya chinensis from 7 provinces (or cities) of China were analyzed using AFLP technique. A total of 336 reproducible bands were ampli- fied with 7 primer combinations from 128 individuals. Two hundred and ninety-two bands (86.90%) were polymorphic. High genetic diversity was found among O. chinensis populations and Wanning population had higher genetic diversity than other po