薏苡仁油注射液对人体肝癌smmc－7721细胞株体外抗肿瘤作用及.pdfVIP

2012 4 20 4 MODERN ONCOLOGY ，Apr. 2012 ，VOI. 20 ，NO. 04 ·693 · 现代肿瘤医学 年 月 第 卷第 期 薏苡仁油注射液对人体肝癌SMMC － 7721 细胞株体外抗肿瘤作用及机 制研究 ， ， ， ， 尹蓓珮 严萍萍 刘 畅 陆宏祺 李炳生 Anti － tumor effect in vitro of coix seed oil injection on the human liver cancer cell SMMC － 7721 and the mechanism study YIN Beipei ，YAN Pingping ，LIU Chang ，LU Hongqi ，LI Bingsheng State Key Laboratory of Innovative Medicines and Pharmaceutical Technology ，Shanghai Institute of Pharmaceutical Industry ，Shanghai 200437 ，China． 【Abstract 】 Objective :To evaluate the anti － tumor effect in vitro of coix seed oil injection on the human liver cancer cell SMMC － 7721 and study the mechanism of action． Methods :Human HCC cells were treated with KLT． CCK － 8 cell viability assay ，scratch assay ，transwell assay and matrigel sprout assay were performed to investigate the effect of KLT on cell proliferation ，migration and invasion． Impact of KLT on apoptosis and cell cycle of cancer cells were measured by flowcytometry． The expression level of target genes of KLT in cancer cells were studied by Western blot． Results :KLT suppressed the cell proliferation ，migration and invasion of human HCC cells． Cell cycle of HCC cells was arrested in G / M phase when treated with KLT and late stage of apoptosis was observed． KLT was 2 capable of inducing the expression of cyclin B1，while down － regulating the expression of cyclin D1，cyclin E． Con- clusion :KLT possesses well acknowledged anti － tumor activity in vitro ，which may result from cell cycle arrest ，ap- optosis ，down － regulation of oncogenes． 【Key words 】coix seed extract (KLT);SMMC － 7721 ;in vitro ;anti － tumor ;mechanism Moder