生物技术毕业论文骆红彦.docVIP

分类号： 密级： 编 号：10299-09-070402-300905032 JIANGSU UNIVERSITY 本 科 毕 业 论 文 The large scale mapping of S12 mutant involved in silique development 学院名称： 食品与生物工程 专业班级： 生物技术1001 学生姓名： 骆 红 彦 指导教师姓名： 刘 天 磊 指导教师职称： 讲 师 2014年 06 月 分类号： 密级： 编 号：10299-09-070402-30 本 科 毕 业 论 文 The large scale mapping of S12 mutant involved in silique development 骆红彦 指导教师 职 称 讲师 专业名称 生物技术 届 别 201届 论文提交日期 论文答辩日期 评阅人 答辩小组组长 答辩委员会主任 很容易进行人工诱变,产生大量突变体separated from F2 generation to confirm the gene’s initial position,in which,the same linear determination is the first step for fine mapping.We use the mixture that is F2 generation mutant and DNA sample to analysis whether the mutant and NGA63 are concatenated,but We need carry out the work that is to measure individual plant genotype to confirm the chain.We plan to use NGA63 to measure the plant genotype of the mutant from F2 generation.Then,We can judge whether the chain between the mutant and NGA63 is true. Next We chose another two molecular markers A8103 and A3925 to measure plant genotype in F2 group.We find that the exchange rates between the S12 mutant and three kinds of molecular markers NGA63, A8103, A3925 are 0%, 3.7%, 0% respectively.The physical distances between NGA63 and A8103，NGA63 and A3925, A8103 and A3925 are 40cM,3cM,35cM respectively. But the practical exchange rates are 6.25%, 0%, 6.25% respectively. From the results,We find the actual detection rate of exchange is lower than the theory.So We suspect that three reasons may lead to this result. Firstly, the number We detected is too low; Secondly, it may be environmental factors.It’s because We don’t carry out the experiment under the optimum conditions,such as the tempe