松针原花青素提取关键技术研究分析.docVIP

毕业设计(论文) 课 题 名 称 学 生 姓 名 学 号 系、年级专业生物与化学工程系0级 指 导 教 师 职 称 2012年 月 日 松针原花青素的提取关键技术研究 史 华 梅 邵阳学院生物与化学工程系08级食品质量与安全(食品科学与工程方向) 湖南 邵阳 422000 指导老师:黄大川 摘 要 本文主要研究了微波辅助提取松针中原花青素的工艺。通过比较3种不同处理方式的松针的吸光度，选定新鲜马尾松松针作为提取工艺研究的原材料。微波辅助提取原花青素具有省时、高效、节能等优点, 且提取物更易于分离纯化。微波水提法的最佳条件：功率500W，时间15min，料液比1：9。用可见光分光光度法对原花青素的含量进行判定，其依据是在其最大吸收波长550nm处，吸光度与原花青素的含量成正相关。通过单因素实验和正交实验，确定微波水提法从松针中提取原花青素的最佳工艺条件：提取温度为100 ℃ , 提取时间为150 m in, pH 为4. 0, 料液比为1：14, 该条件下原花青素的吸光度为10.566。 关键词：松针；原花青素；微波辅助水浸提法； Study on optimum process for extraction of proanthocyanidin from pine needles By： SHI Huamei Department of Biology and Chemistry Engineering,Shaoyang University,Shaoyang Hunan 422000,China Directed by:HUANG Dachuan ABSTRACT This paper is a study on the optimum process for extraction of Proanthocyanidins from pine neddles.By comparing the absorbance of three different pine neddles which are dealt with three different ways, we finally choose the fresh pine neeles as the raw materials to study the extraction process of Proanthocyanidins. Microwave-assisted extraction of Proanthocyanidins from pine neddles is time-saving, efficient and energy-saving. The optimum conditions of extraction are the rate of solution to solid 9: 1, the power 500W, extraction time 15 minutes.Using spectrophotometry to determine the content of Proanthocyanidins from pine neddles in the extracts on the basis of the absorbance Proportional to the content of Proanthocyanidins under the maximum absorption wavelength 550nm.By single factor and orthogonal experiment the best extraction process conditions was confirmed: extraction temperature of 100℃, extracting time for 150min, pH = 4.0, the ratio of solid to liquid is 1 : 14.The absorbance is 10.566 under such conditions. Key words: pine neddles; Proanthocyanidins; Microwave-assisted extraction  目录 摘 要 I ABS