金荞麦叶保护酶与保护物质对增强UV-B辐射及干旱胁迫处理的响应资料.docVIP

金荞麦叶保护酶与保护物质对增强UV-B辐射及干旱胁迫处理的响应 陆翔，张益锋 (绍兴文理学院元培学院生命科学系，浙江 绍兴312000) [摘要] 目的：研究金荞麦叶保护酶与保护物质对增强UV-B辐射及干旱胁迫处理的响应，为其规范化种植提供科学依据。方法：采用盆栽试验的方法，测定金荞麦在干旱和UV-B辐射胁迫下超氧化物歧化酶 (SOD) 活性过氧化物酶 (POD)活性总黄酮含量游离脯氨酸UV-B辐射提高了金荞麦叶SOD活性、总黄酮含量、游离脯氨酸含量；增强的UV-B辐射在处理前期和后期提高了金荞麦POD活性；增强的UV-B辐射在处理前期增加了金荞麦叶可溶性蛋白含量。在干旱胁迫下，增强的UV-B辐射在处理中期提高了金荞麦SOD活性，而在处理前期和后期却降低了其SOD活性；增强的UV-B辐射在处理后期升高了金荞麦叶POD活性；提高了金荞麦叶游离脯氨酸和可溶性蛋白含量。结论：在干旱条件下，金荞麦通过酶系统和保护物质的调节减少胁迫带来的不利影响，增强试验设定强度的UV-B辐射在一定程度上有利于提高其对干旱的抗性。 [关键词] 金荞麦；干旱胁迫；增强UV-B辐射；酶系统；保护物质 Response of the protective enzymes and protective substances of Fagopyrum dibotrys under enhanced UV-B radiation and drought stress LU Xiang,ZHANG Yifeng (School of Life and Sciences, Yuanpei College Shaoxing University, Shaoxing 312000, China) [Abstract] Objective: To study the influences of enhanced UV-B radiation in simulation and drought stress on the protective enzymes and protective substances of Fagopyrum dibotrys, and so as to provide a theoretical basis for the cultivation for F. dibotrys. Method: By experiment of potted plant, we mensurated the SOD activity, POD activity, total flavonoids contents, free proline contents and soluble protein contents of F. dibotrys under different drought stresses and UV-B radiations. Result: In conditions of adequate water, enhanced UV-B radiation increased SOD activity, total flavonoids contents and free proline contents of F. dibotrys. In the early and late stages of the treatment, POD activity of F. dibotrys was increased by enhanced UV-B radiation. In the early and late stages of the treatment, soluble protein contents of F. dibotrys was increased by enhanced UV-B radiation. In conditions of drought stress, enhanced UV-B radiation increased SOD activity of F. dibotrys in the medium-term of the treatment while decreased it in the early and late stages of the treatment. In the late stage of the treatment, enhanced UV-B radiation increased POD activity, free proline contents and soluble protein contents of F. dibotrys. Conclusion: In con