MU OPIOD RECEPTOR iS INVOLVED IN THE ANALGESIC EFFECT OF DOCOSAHEXAENOIC ACID IN MICE.pptVIP

——临床医学07级 报告人：岑志栋 组员：胡瑞、潘文珏、钟勋、陈立、曹圳健、苏杰 二十二碳六烯酸（DHA）对小鼠的镇痛作用与?阿片受体有关MU OPIOD RECEPTOR iS INVOLVED IN THE ANALGESIC EFFECT OF DOCOSAHEXAENOIC ACID IN MICE BACKGROUND: BACKGROUND: Dietary supplementation with selective n-3 fatty acids would be most beneficial for the treatment of pain[1] Omega-3 PUFAs are an attractive adjunctive treatment for joint pain associated with rheumatoid arthritis, inflammatory bowel disease, and dysmenorrhea[2] TRPV1 is a novel target for omega-3 polyunsaturated fatty acids[1] Analgesic effects of DHA were abolished when mice were pretreated with naloxone, an opioid receptor antagonist[3] SIGNIFICANCE: It’s a new step in the research of DHA’s analgesic effect. It will give us more information about the mu opiod receptor-mediated pain control mechanism of DHA, which will help us take a step forward on the way to find new analgesics. Innovation ：The first study of exogenous DHA on the regulation of opioid receptor expression and its temporal and spatial characteristics METHOD: Intracerebral injection Hot-plate test and torsion test Western blot RT-PCR Immune-Histochemical INTRACEREBRAL INJECTION The injection was performed as previously described [4]. Drugs were administered through a 27 gauge BD*Yale* reusable hypodermic needle, connected to a 50 μl Hamilton gastight syringe with a repeating dispenser. The needle was inserted into the lateral ventricle of mouse brain through the skull. A certain volume was used in each group. PRE-EXPERIMENT: HOT-PLATE TEST DHA 1μl DHA 5μl[5] DHA 10μl 30mins START! Hot-plate test Get the best dose and its time-response relationship 15 30 60 90 120 150 180 (min) N=10/group Morphine 5μl[5] HOT-PLATE TEST DHA Control Morphine 5μl[5] 30mins START! Hot-plate test Compare the analgesic effect and time-response relationship between DHA and morphine 15 30 60 90 120 150 180 (min) N=10/group TORSION TEST: DHA Control