玫烟色棒束孢侵染对小菜蛾幼虫体内不同酶活的影响.pdfVIP

菌物学报 jwxt@ 15 March 2013, 32(2): 269-276 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q © 2013 IMCAS, all rights reserved. 玫烟色棒束孢侵染对小菜蛾幼虫体内不同酶活的影响 1 2 3 3﹡ 王宏民 张奂 郝赤 张仙红 1 山西农业大学经济与贸易学院 山西 太谷 030801 2 运城高等师范专科学校 山西 运城 044000 3 山西农业大学农学院 山西 太谷 030801 摘 要：在实验室条件下，测定了昆虫病原真菌玫烟色棒束孢Isaria fumosorosea 的侵染对小菜蛾Plutella xylostella 3 龄幼虫体内酚氧化酶（PO ）和不同抗氧化酶类，包括超氧化物歧化酶（SOD）、过氧化物酶（POD ）、过氧化氢（CAT ） 及谷胱甘肽S-转移酶（GSTs ）活力的变化。结果表明，玫烟色棒束孢侵染小菜蛾3 龄幼虫后，体内PO 、SOD、POD 、 CAT 和GSTs 活力均受到明显的影响。其中感病小菜蛾幼虫体内的PO 活力始终高于同期未感染的小菜蛾幼虫，当接 菌40h 时，酚氧化酶活力达到最大37.4U/g，为对照的2.6 倍，而SOD、POD 、CAT 和GSTs 活力在感病前期明显高 于对照，接菌40–48h 各种酶活力均达到最大，当接菌56h 酶活力开始下降，64h 时酶活力均明显低于对照。可见玫 烟色棒束孢的侵染严重干扰了小菜蛾幼虫体内正常的生理活动。 关键词：玫烟色棒束孢，小菜蛾幼虫，酚氧化酶，抗氧化酶 Effects of Isaria fumosorosea infection on different enzyme activities in the larvae of Plutella xylostella 1 2 3 3* WANG Hong-Min ZHANG Huan HAO Chi ZHANG Xian-Hong 1College of Economics and Trade, Shanxi Agricultural University, Taigu, Shanxi 030801, China 2 Yuncheng Teachers College, Yuncheng, Shanxi 044000, China 3College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China Abstract: The activities of phenoloxidase (PO) and different antioxidative enzymes, including superoxide dismutase (SOD), perioxidase (POD), catalase (CAT) and glutathione S-transferase (GST) in the 3rd instar larvae of diamondback moth (Plutella xylostella) infected by Isaria fumosorosea were studied. The results showed that activities of PO and antioxidative enzymes in the 3rd instar larvae were significantly affected by I. fumosorosea. PO activity in the infected larvae was always higher than that in the control, and reached a value of 37.4U/g, being 2.