EfficientSynthesisof(S)-1-(2-chlorophenyl)ethanolintheSubmergedCultureofAlternariaalternataIsolate.pdfVIP

第 30 卷 第 4 期 催 化 学 报 2009 年 4 月 Vol. 30 No. 4 Chinese Journal of Catalysis April 2009 文章编号: 0253-9837(2009)04-0370-05 研究论文: 370~374 Efficient Synthesis of (S)-1-(2-chlorophenyl)ethanol in the Submerged Culture of Alternaria alternata Isolate 1,* 1 2 3 Esabi B. KURBANOGLU , Mesut TASKIN , Kani ZILBEYAZ , Ismet HASENEKOGLU 1 Department of Biology, Faculty of Arts and Sciences, Ataturk University, 25240 Erzurum, Turkey; 2Department of Chemistry, Faculty of Arts and Sciences, Ataturk University, 25240 Erzurum, Turkey; 3Department of Biology, Faculty of Kazim Karabekir Education, Ataturk University, 25240 Erzurum, Turkey Abstract: (S)-1-(2-chlorophenyl)ethanol is a key intermediate of L-cloprenaline used for relieving asthma symptoms. The asymmetric re- duction of 2-chloroacetophenone 1 to (S)-1-(2-chlorophenyl)ethanol 2 in the submerged culture of Alternaria alternata isolates was studied. A. alternata EBK-8 isolate was the most effective biocatalyst. The bioactivity of the fungus could be significantly improved by the optimiza- tion of culture conditions. Parameters such as pH, temperature, agitation, and incubation time considerably influenced the substrate conver- sion and the optical purity of the product. The reaction was carried out in a culture medium at a substrate concentration of 30 mmol/L and produced the desir