利用SSR分析红菜苔的遗传多样性.pdfVIP

植物遗传资源学报2012,13(6):1088-1092 Journal of Plant Genetic Resources 利用SSR分析红菜苔的遗传多样性 1 1 1 1 2 周晓波 ,白占兵 ,丁茁荑 ,吴艺飞 ,王晓武 1 2 ( 湖南省蔬菜研究所,长沙410125; 中国农业科学院蔬菜花卉研究所,北京 100081) 　 　 摘要:红菜苔是我国独有的蔬菜资源,深受消费者喜爱。 利用63 对SSR 引物检测来自全国的45份红菜苔种质资源的 遗传多样性,63 对引物扩增出124 条带,平均每条引物扩增出近2 条带,扩增产物片段大小都在 150 ~300bp之间,相似系 数在0．56 ~0．89之间。 结果表明,红菜苔具有丰富的遗传多样性,本研究为菜苔资源的利用和育种提供了分子生物学 依据。 　 　 关键词:红菜苔;种质资源;SSR;遗传多样性 Genetic Diversity of Tsai-tai Germplasm Revealed by SSR Markers 1 1 1 1 2 ZHOU Xiao-bo ,BAI Zhan-bing ,DINGZhuo-yi ,WU Yi-fei ,WANG Xiao-wu 1 2 ( Hunan Vegetable Research Institute,Changsha,410125;Institute of Vegetables and Flowers,ChineseAcademy of Agricultural Sciences,Beijing 100081) Abstract:Tsai-tai is a vegetable resources of our unique,loved by consumers.SSR methods were applied to detect genetic diversity of 45 Tsai-tai germplasm from China,provided biological foundation for the utilization and seed breeding of the Tsai-tai germplasm.124 bandswere amplified with 63 SSR primers.The amplified DNA frag- ments size were 150-300bp.Similarity coefficientsbetween0．56-0．89.Theresultsshow therewereabundantge- netic diversity in the Tsai-tai germplasm of China.This provides the basis for molecular biology research for the Tsai-tai resource utilization and breeding. Key words:Tsai-tai;Germplasm;SSR;Genetic diversity 红菜苔(Brassica campestris L.ssp.chinensis var. 年利用RAPD对紫菜苔自交系染色体 DNA进行了 perperea Hort.)别名紫菜苔、红油菜苔,是十字花科 [13] 研究,李焰焰等 对紫菜苔快速碱化因子基因Bc- 芸苔属