利用分子标记将2Ai-2染色体转移到小麦ph1b遗传背景的研究.pdfVIP

植物遗传资源学报2012,13(5):838-842 Journal of Plant Genetic Resources 利用分子标记将2Ai-2染色体转移到 小麦ph1b遗传背景的研究 雷　 昊,张　 悦,林志珊,徐琼芳,叶兴国 (中国农业科学院作物科学研究所/ 农业部作物遗传育种重点开放实验室/ 国家作物基因资源和基因改良重大科学工程,北京 100081) 　 　 摘要:小麦ph1b 突变体可诱导部分同源染色体配对和交换,产生遗传上较为稳定、补偿性较好的重组体。 将外源染色体 引入ph1b 的小麦遗传背景是产生目标染色体重组体的基础,但ph1b植株没有明显而稳定的表型性状,难以从表型上进行选 择。 本研究利用CSph1b缺失区中的分子标记Mads及外源染色体特异的分子标记P4和P68,对小麦-中间偃麦草2Ai-2(2B ) 异代换系N420 与CSph1b 的杂种F 群体及其衍生的F 株系进行 ph1b-2Ai-2 染色体综合体的选择,高效地获得了目标基 2 5 因型。 　 　 关键词:小麦;分子标记;ph1b;外源染色体;部分同源染色体 Transferring of the2Ai-2 Chromosome into Wheat ph1b Genetic Background by Using Related Molecular Markers LEI Hao,ZHANG Yue,LIN Zhi-shan,XU Qiong-fang,YE Xing-guo (NationalKey Facilityfor Crop Genetic Resources and Genetic Improvement/ Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/ Institute of Crop Sciences,ChineseAcademy of Agricultural Sciences,Beijing 100081) 　 　 Abstract:Incident ofhomeologouschromosomepairingandexchangeoftenoccurinhexaploidwheatwithph1bge- netic backgroundwhich canproduce compensable and genetic stable recombinants.Therefore,transferring alien chromo- somesinto awheat material with ph1b background is the basis of generating translocations between the target chromo- somes.However,it isdifficult to identify ph1b genotype according to its phenotype.In present study,some target plants withph1b andthe alien chromosome2Ai-2were selected effectively by using molecular markers Mads (on the deletion region of ph1b)for ph1b,P4 andP68 (onthealienchromosome)for2Ai-2inthe F populationof CSph1bcrossedwith 2 N420,awheat-Thinopyrum intermedium2B(2Ai-2)substitutionline,andtheir derivative F lines.