利用热稳定蛋白特异条带鉴别籼粳稻的方法研究.pdfVIP

2011, 12( 1): 1924 Journal of P lant Genetic Resources 利用热稳定蛋白特异条带鉴别籼粳稻的方法研究 付深造, 辛 霞, 张志娥, 陈晓玲, 辛萍萍, 卢新雄 (, 100081) : 以 85份栽培稻为材料, 利用 SDS聚丙烯酰胺凝 电泳和蛋白免疫印迹技术分析籼稻和粳稻热稳定蛋白的表达差 异, 探讨利用特异蛋白来建立籼粳稻的鉴别方法结果表明, 在籼粳稻品种之间存在热稳定蛋白的差异表达, 尤其在分子量 约 42~ 47 D 范围其中45. 2 D条带 ( Band ) 和 465 D( Band ) 条带为典型粳稻特异蛋白 ( Os03g0168100 )的标志带, 420 D条带 ( Band ! )为典型籼稻特异蛋白 ( OsI_10172)的标志带以这 3条带作为鉴别方法, 并与程氏指数法进行比较, 典型籼稻和粳稻的一致度分别为 800% 和 864% , 表明热稳定蛋白标志带法在 一定程度上可用于鉴别典型的籼稻和粳稻 : 籼稻; 粳稻; 热稳定蛋白; SDS聚丙烯酰胺凝 电泳; 蛋白免疫印迹 Method for IdentifyingInd icaJap on ica Rice by Using SpecificHeatStable Protein Bands FU Shenzao, X IN X ia, ZHANG Zh ie, CHEN X iaoling, X IN Pingp ing, LU X inxiong (Institu te of Crop Sciences, Chinese A cademy of Ag r icultural Sciences, Beij ing 100081 ) Abstract:D ifferential expression of heatstable proteins in eightyfive cu ltivated rice(Ory a sa tiva L. ) w as an alyzed by using SDSPAGE andw estern blotting, to explore the feasib ility of using specific proteins to identify Indi caJap on ica rice. The results show ed that the heatstable proteins expressed d ifferentially betw een Ind ica and Ja p onica rice, especially in the molecu lar w eight of about 4247 D range. Among them, 45. 2 D band( Band ) and 46. 5 D band( Band ) w eremar er bands for typicalJap onica rice. Both bandsm atched to Os03g0168100 in NC BI protein databan , 42. 0 D band( Band !, m atched to OsI_10172) w asmar er band for typ icalIndica rice. E ight yfive Indica andJap on ica rice varietiesw ere identified based on the expression pattern of these three bands, and then compared the resu ltsw ith Cheng∀ s index. The concordancesw ere 800% and 864% in typical Ind ica rice and Jap onica rice, respectively. Therefore, the method of heststable protein mar er bands can be used for identifying typical Indica rice andJap onica rice.