九台晚李PGIP基因的克隆及生物信息学分析.pdfVIP

植物遗传资源学报2010。11(5)：650-653 ofPlantGeneticResources Journal 九台晚李尸饼P基因的克隆及生物信息学分析 郭庆勋，张春雨，王晶莹，周连霞，王彦涛 (吉林大学植物科学学院，长春130062) 氨基酸，其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列分析表明：该基因与中国李、杏、桃、马哈利樱桃、梅等 李属植物的JPc，P基因序列一致度迭95％一99％。系统进化分析显示出属内亲缘关系较近、属间亲缘关系较远的特点。该序 列为植物分子抗病育种提供了1务新的基因资源。 关键词：九台晚李；PGIP基因；基因克隆；生物信息学 andBioinformaticofPGIPGenefrom Cloning Analysis Jiutaiwanli GUO Yah—tao Qing—xun，ZHANGChun—yu，WANGJing-ying，ZHOULan—xia，WANG ofPlantScience，Jilin (College Univers毋，Changchun，130062) withafull of1192 with Abstract：A accession：GU068978)was targetfragment length bp(GenBank amplified asthe andtheconservativeofPGlP DNAofPnl／／,／2,$salwinaL．CV．Jiutaiwanli、eaves genomic templates sequences asthe hadafull frame the protein， gene primers．Thissequence openreadingencoding andcontainedtwoexons oneintron．Thetotalexonswere of enco— interruptedby comprisedby990bpdeoxynucleotide 330aminoacid．A leucine-rich existedinthederived anal— ding conserved fragmenthad proteinsequence．Sequencing of showedthatitwas95％to99％identicalwiththe PrunusPGlp P．salicina．P．c盯一 ysis sequences genesincluding that the wascloser treeshowed within geneticrelationship genus raeniaca，P．persica，P．mahaleb，P．m／Lme．Phylogenic andbet