蛋白芯片载体表面修饰对探针固定影响研析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 10 5 1 2 3 2 2 1 王涛 郭子瑜 张瑞 聂利利 贾芸芳 邢克礼 1 2 ( , 300070; , 300071; 3, 300070) : 采用 3氨基丙基三甲氧基硅烷( ( 3am inopropyl) trmi ethoxysilane, APTES)戊二醛( gl taraldehyde, GA)多聚L 赖氨 ( polyLlysine, PLL) 修饰芯片载体表面, 对 3种不同修饰方法制备的蛋白质芯片进行对比研究将 Cy3标记羊抗鼠 IgG 固定在修饰后片基上, 选择蛋白探针的固定率作为检测指标; 将小鼠 IgG作为探针固定在芯片上, 靶蛋白为 Cy3标记羊抗 鼠 IgG, 通过生物芯片扫描仪检测反应后荧光强度, 选择蛋白探针的反应性作为检测指标, 探讨制备蛋白质芯片较佳的表面修 饰方法结果显示, 戊二醛修饰玻片对蛋白固定较好, 有较高的反应活性, 检测限较宽, 但背景噪声较高 : 蛋白质微阵列 表面修饰 固定率 反应性 E ffect of SurfaceM odification for ProteinM icroarray on Imm obilizing P rotein Probe 1 2 3 2 2 1 W ang T ao G o Ziy Zhang R i N ie Lili JiaY nfang X ing Keli 1 ( School of B iomedical Engineering, Tianj inM edical University, Tianj in 300070; 2 College of Inf ormation Technical Science,N ankai University, Tianj in 300071; 3 Tianj in M edical University M eta olic D iseasesH osp ita,l Tianj in 300070) Abstrac:t G lass slidesw erem odified w ith( 3am inopropyl) trmi ethoxys ilane, gl taraldehyde, polyLlysine to prepare protein m i croarrays. The optmi al s rfacem od ification m ethodsw ere eval ate and chosen for the preparation of protein m icroarray. The mi m ob ilizing proteinsw ere labeled by Cy3, and attachm ent ratew as eval ated. Then, themo se IgG w ere prin ted on themod