微流控芯片上胰岛素样生长因子1和碱性成纤维细胞生长因子对兔关节软骨细胞增殖的影响.pdfVIP

　 军 杂 20 13年6月1 日　第38卷　第6期 476 解放 医学 志　 论　著 微流控芯片上胰岛素样生长因子1和碱性成纤维细胞生长因 子对兔关节软骨细胞增殖的影响 李元城，张卫国，秦建华，林炳承 [摘要]　目的　在微流控芯片平台上探索三维培养环境下胰岛素样生长因子1(I GF- 1)和碱性成纤维细胞生长因子 (bFGF)对软骨细胞体外增殖的影响。方法　微流控芯片上，分别在I GF- 1、bFGF 以及二者组合浓度梯度下三维培养兔 关节软骨细胞，2周后计算软骨细胞增殖率并与传统的96孔板培养结果进行比较。结果　I GF- 1在57. 14ng/ml产生最大 增殖效应，平均倍增2.38倍；bFGF在5.72ng/ml产生最大增殖效应，平均倍增3.85倍；85.7 1ng/ml IGF- 1+ 1.43ng/ml bFGF 的组合可使软骨细胞产生最大的增殖效应，平均倍增4 .76倍。微流控芯片与96孔板传统培养的软骨细胞增殖率差异无 统计学意义。结论　一定浓度组合的I GF- 1和bFGF可协同产生最大的增殖效应；微流控芯片技术可用于软骨组织工程 领域，具有广阔的应用前景。 [关键词]　软骨细胞；组织工程； 微流控芯片；胰岛素样生长因子1 ；成纤维细胞生长因子2 [中图分类号]　R394.26 [文献标志码]　A [文章编号]　0577-7402 (20 13)06-0476-05 Effects of insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) on the proliferation of rabbit articular chondrocytes embedded in microfluidic chip LI Yuan-cheng 1 1* 2 2 , ZHANG Wei-guo , QIN Jian-hua , LIN Bing-cheng 1Department of Orthopedics, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116001, China 2Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116001, China * Corresponding author, E-mail: zhxiao_2004@ [Abstract]　Objective　To inv estigate th e effects of insulin-like grow th factor- 1 (IGF- 1) and basic fibroblast grow th factor (bFGF) on th e proliferation of ch ondrocy tes in a 3-dimensional (3D) culture env ironment of microfluidic chip. Method　R abbit articular ch ondrocy tes emb edded in microfluidic chip and 96 -w ell plate w ere cultured w ith solitary and comp onent concentrations of I GF- 1 and bFGF for 2 w eek s, and th en th e proliferation rates of ch ondrocy tes w ere cal cul ated and compared. Results　Th e max imal proliferation effect of IGF- 1 w as found wh en th e concentration reac