土壤杆菌ATCC31749合成可德胶研析进展.pdfVIP

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN 年第 期 2013 3 土壤杆菌 ATCC31749 合成可德胶的研究进展 余小琴  吴毅歆  何月秋  毛自朝 （云南农业大学农学与生物技术学院，昆明 650201） 摘 要 ： 可德胶是一种具独特凝胶性质，由β-1，3-糖苷键连接的中性多糖。近年来随着食品、化工、 医药等产业的发展， 对可德胶需求也逐年增长。土壤杆菌ATCC31749 是可德胶合成的优良发酵菌。结合已测序的基因组精细图和蛋白组学研究，从可 德胶多糖合成的生物合成途径与能量代谢、信号转导及其调控机制等的研究进展来阐明可德胶发酵生产过程中产量形成的主要限 制因素，并据此提出更高效可德胶生产菌株改良及发酵生产条件优化的策略与手段。 关键词 ： 基因组精细图和蛋白组学 可德胶生物合成 调控机制 Research on Curdlan Synthesized by Agrobacterium sp. ATCC31749 Yu Xiaoqin Wu Yixin He Yueqiu Mao Zichao （ ， ，K 65020 1 ） Agronomy and Biotechnology Institute Yunnan Agriculture University unming Abstract:  Curdlan is a high molecular β-1, 3-glucan with unique gel nature. Curdlan and it ’s modified molecule were used as new materials applied widely in industries of food, pharmaceutics and chemical engineering, resulting in the growing of curdlan demand year by year. Agrobacterium sp. ATCC31749 is an efficient industrious strain for curdlan production. This review summarized the advance of the fermentation and physiology of curdlan biosynthesis of the strain. By combining with ATCC31749 genomic information and proteomics study, we focus on signal transduction and regulation, energy producing and metabolic pathway of this special polysaccharides biosynthesis ；then analyze the restriction factor for curlan synthesis and propose the strategies for how to improve the stra