白地霉遗传多样性及生态地理起源地的相关性分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2011 5 刘天明 王会会 王娟娟 (, 250353) : 采用随机扩增多态性DNA(RAPD)分子标记方法从 22 个随机引物中筛选出 TubeB03TubeB07TubeB12三 个最 引物分析来源于 7 个省区的 23株白地霉的种内遗传多样性, 并用 UPGMA聚类分析法评价它们之间的亲缘关系结 果表明, 不同来源地的白地霉通过 RAPD分析显示出较高的遗传差异性,并且, 在系统进化树上处于同一分枝的菌株来源于生 态地理相近的区域, 仅有 个别例外因此, 应用 RAPD分子标记技术对来自不同生态区的白地霉种内遗传多样性和亲缘关系 进行分析是可行的 : 白地霉 RAPDPCR 种内遗传多样性 亲缘关系 R elationship Analysis Between G enetic D iversity ofG eotrichum cand idum and G eographical Regions Liu Tianm ing Wang uihui Wang Juanjuan (Colleg e of F ood and B ioeng ineering, Sha nd ong Institute of L igh t Ind ustry, J ina n 250353) Abstrac:t The genetic diversity of 23Geo trichum cand id um strains from 7 provinceswere studiedw ith random amplified polymor phism DNA (RAPD) analysis by using 22 random prmi ers. The best three prmi erswereTubeB03, TubeB07, TubeB12. The genetic relationship among 23 strainswas analyzed by UPGMA method. The result showed that therewas genetic diversity bewt een strains from different regions, and the strainswhich shared the same cluster on thephylogenetic tree originated from smi ilar ecologicalgeography are as, only therewere a few exceptions. ence, RAPD is feasible on analyzing genetic diversity and genetic relationship ofGeo trichum can d id um strains from various ecological regions. K ey w ords: Geo trich um cand id um RAPDPCR Intraspecific genetic diversity Genetic relationship (G eotrichum cand id um ) DNA PCR , , ,, 23 ,, DNA RAPD , , , RAPD [ 1] DNA(RAPD) ,