基于高通量测序技术的细菌非编码RNA的研究方法进展.pdfVIP

生物技术通报 ·特约综述· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 4 :99-104 基于高通量测序技术的细菌非编码 RNA 研究方法进展 郭明璋  许文涛 （中国农业大学食品科学与营养工程学院 食品安全与分子生物学实验室，北京 100083） 摘 要 ： 细菌非编码RNA 指细菌中不编码蛋白质，而以RNA 的分子形式起调控作用的一类核酸分子。高通量测序技术的 应用极大地推进了多种细菌中非编码RNA 的发现工作，但由于现阶段对细菌非编码RNA 特征的认识尚不够深入，该领域测序 数据的生物信息学分析还存在许多不足。介绍了应用高通量测序技术研究细菌非编码RNA 的两种主要技术——RNA-seq 技术和 dRNA-seq 技术，对现行的筛选非编码RNA 的生物信息学分析方法进行综述，并对该领域生物信息学分析策略的改进提出设想。 关键词 ： 细菌；非编码RNA ；高通量测序；生物信息学 DOI ：10.13560/ki.biotech.bull.1985.2015.03.015 Progress in Methodology of Bacterial Noncoding RNA Research Based - on High throughput Sequencing Guo Mingzhang Xu Wentao （Laboratory of Food Safety and Molecular Biology ，College of Food Science and Nutritional Engineering ， China Agricultural University ，Beijing 100083 ） Abstract: Bacterial noncoding RNA refers to the nucleic acid molecules that do not encode proteins, but play roles in the regulation in the form of RNA in bacteria. Application of high-throughput sequencing technology has greatly advanced the discovery of non-coding RNA in a variety of bacterial species. However, due to the lack of enough knowledge on the characteristics of non-coding RNA in bacteria, the bioinformatics analysis of the sequencing data are still defective. This article describes two main techniquesthat applied high-throughput sequencing to be used in the researches on bacterial non-coding RNA ：RNA-seq and dRNA-seq. The existing bioinformatics methods for screening of non-coding RNA are reviewed, and the recommendations for improving the bioinformatics strategy in this field are