克咳片对慢性阻塞性肺疾病老年大鼠模型的实验研究.pdfVIP

第十一届中南地区实验动物科技交流会论文集 克咳片对慢性阻塞性肺疾病老年大鼠模型的实验研究 严家荣，钟志勇，黄小琼，刘盛来，邝少松 （广东省医学实验动物中心，佛山 528248 ） 【摘要】目的 观察克咳片对慢性阻塞性肺疾病老年大鼠模型的治疗作用。方法 将 116 只 SD 老年大鼠随机分为正常对照组 10 只和模型组 106 只，采用烟熏联合脂多糖法联合造 模，试验第 31 天，将存活的模型动物随机分为模型对照组、通宣理肺片组、克咳片高剂量 组、克咳片中剂量组、克咳片低剂量组，克咳片超微粉组，每天给予烟熏及治疗，连续 30 天。治疗结束后。检测各组动物肿瘤坏死因子 α （TNF-α）、白三烯(LTC4)含量、肺功能情 况及肺病理组织学变化。结果 与模型组相比，克咳片高剂量可以降低 TNF-α含量，延长呼 吸峰值，降低气道阻力，降低肺炎症细胞浸润程度；克咳片超微粉可以降低 TNF-α 含量， 延长呼吸峰值，降低气道阻力，降低肺气肿程度。结论 克咳片对慢性阻塞性肺疾病老年大 鼠模型具有一定的改善作用。 【关键词】克咳片；慢性阻塞性肺疾病；动物模型 Experimental study of Kekepian on chronic obstructive pulmonary diseases model in aged rats YAN Jia-rong, ZHONG ZHi-yong, HUANG Xiao qiong LIU Sheng lia KUANG Shao song (Guangdong Medical Laboratory Animal Center, foshan, 528248) 【ABSTRACT 】Objective: To observe therapeutic effect of Kekepian on chronic obstructive pulmonary diseases. Method: 116 aged SD rats were randomly divided into two groups: 10 rats for control and 106 for disease model group. Disease model was established by smoking combined with lipopolysaccharide for 31 days and the rats of disease model were divided into five groups: disease model control group, Tongxuanlifeipian group, Kekepian group with high dosage, Kekepian group with medial dosage, Kekepian group with low dosage ，Kekepian in ultrafine powder. Rats were treated with smoking and drugs for 30 days. Examine the level of TNF-α and Leukotriene, states of lung function and histopathology changes of the lung. Result: Compared to the disease model group, Kekepian group with high dosage can decrease the level of TNF-α, prolong respiratory peak, reduce airway resistance and the degree of emphysema.Conclusion: Kekepian can improve chronic obstructive pulmonary diseases in aged