参附注射液对阿霉素所致心衰大鼠氧化应激水平及血管紧张素Ⅱ的影响.docVIP

参附注射液对阿霉素所致心衰大鼠氧化应激水平及血管紧张素Ⅱ的影响 　　[摘要] 目的 探讨参附注射液对阿霉素（ADR）所致心衰大鼠氧化应激水平及血管紧张素Ⅱ（AngiotensinⅡ）的影响。 方法 将90只大鼠随机分为6组：正常对照组、模型组、卡托普利组（作为阳性对照），参附注射液低、中、高剂量组，每组各15只。正常对照组仅给予等体积生理盐水，模型组于实验开始后第2、4天腹腔注射ADR 1 mg/kg，第6、8天腹腔注射ADR 2 mg/kg，第10、12天腹腔注射ADR 3 mg/kg，第14、16天腹腔注射ADR 4 mg/kg，累计用药剂量达20 mg/kg。卡托普利组于实验开始后第5天卡托普利180 mg/（kgd）灌胃。参附组于造模后1周，按低、中、高剂量组分别腹腔注射参附注射液1、2、4 mL/kg，每日1次，共2周。处理结束后测定心肌超氧化物歧化酶（SOD）活力和心肌丙二醛（MDA）含量；测定血清中Angiotensin Ⅱ水平。光镜观察心肌病理形态学变化；电镜观察心肌超微结构变化。 结果 阿霉素能显著降低大鼠心肌SOD活性，卡托普利、4 mL/kg参附注射液可将心肌中SOD活性升高至（125.26±7.21）、（128.44±10.02）U/L，与模型组比较差异有高度统计学意义（P 中国论文网 /6/view-7218227.htm 　　[关键词] 参附注射液；阿霉素；血管紧张素Ⅱ；心力衰竭；超氧化物歧化酶 　　[中图分类号] R332 [文献标识码] A [文章编号] 1673-7210（2016）01（a）-0021-04 　　Effects of Shenfu Injection on oxdative stress and AngiotensinⅡ of rats with heart failure induced by Adriamycin 　　ZHANG Zhigang SUN Guodong CHANG Wenhua CHEN Jianzhong▲ 　　Department of Pharmacology， Liaocheng People’s Hospital， Shandong Province， Liaocheng 252000， China 　　[Abstract] Objective To explore the effects of Shenfu Injection on oxdative stress and angiotensinⅡof rats with heart failure induced by Adriamycin. Methods Totally 90 rats were randomly divided into five groups with 15 rats in each group： normal control group， model control group， Captopril group （as positive control）， and Shenfu Injection low， medium， high dose group. The normal control group was only treated with saline by intraperitoneal injection throughout the course of the experiment， while the other four groups were given Adriamycin by intraperitoneal injection with the total dosage of 20 mg/kg. For the Shenfu Injection low， medium， high dose group， 1， 2， 4 mL/kg Shenfu Injection were given after Adriamycin Injection seven days later. For the positive control group， 180 mg/（kgd） Captopril was given. After the model was successfully established， relevant indexes were measured. The activities of plasma superoxide dismutase （SOD） and the contents of malondialdehyde （MDA） in myocardial tissues were measured. The leve