蜂胶对大鼠皮肤创伤愈合的影响.docVIP

蜂胶对大鼠皮肤创伤愈合的影响 　　[摘要] 目的 探讨蜂胶对创伤愈合的影响，并初步探讨创伤愈合机制。 方法 60只健康雄性Wistar大鼠随机分为实验组（创伤模型+蜂胶组）20只、对照组（创伤模型+碘伏组）20只、空白组（创伤模型+生理盐水组）20只，每组再随机分为5个时间点（3、5、7、10、14 d），每组每时间点各4只，采用手术法复制大鼠背部皮肤全层1 cm×1 cm的创伤模型，每日换药。分别于各个时间点处死大鼠，切取创面皮肤组织，计算创伤愈合率，通过HE染色观察创面组织学的变化，免疫组织化学染色法检测血管内皮生长因子（VEGF）和血小板源性生长因子（PDGF）的动态改变。 结果 与对照组比较，实验组能明显提高大鼠创伤愈合速度和愈合率（P中国论文网 /6/view-7221696.htm 　　[关键词] 蜂胶；碘伏；创伤愈合；血管内皮生长因子；血小板源性生长因子；中草药 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1674-4721（2016）01（c）-0011-04 　　[Abstract] Objective To discuss the influence of propolis on wound healing and study preliminarily on the mechanism of wound healing. Methods 60 healthy and male Wistar rats were randomly divided into experimental group （wound model+propolis，n=20），control group （wound model+povidone iodine，n=20） and blank group （wound model+physiological saline，n=20）.Each group was then randomly divided into 5 time points：3 d，5 d，7 d，10 d and 14 d with there were 4 rats in each time point.The wound model was established by full-thickness skin injury in rats′ back in size of 1 cm×1 cm，changed dressings every day.The rats were executed in every time point and the injuried skin tissues were cut to calculate the wound healing rate，and the change of wound histology was observed by hematoxylin-eosin （HE） staining and the dyanmic change of vascular endothelial growth factor（VEGF） and platelet-derived growth factor（PDGF） was detected by immunohistochemical staining method respectively. Results Compared with control group，the experimental group could obviously improve the healing speed and healing rate of wound in rats （P 　　[Key words] Propolis；Povidone-iodine；Wound healing；Vascular endothelial growth factor；Platelet-derived growth factor；Chinese herbal medicine 　　蜂胶的药用价值极高，其化学成分包括黄酮类化合物、烯、萜类化合物及大量微量元素等300多种成分，已被证实具有降血糖、抗菌、免疫增强、抗炎，抗氧化等多种药理作用[1-4]，其中抗菌与抗炎作用为创伤愈合提供了药理基础。近年来，国内外已有部分关于蜂胶促愈合的研究，而在临床治疗过程中，碘伏仍是外科创伤的首选消毒、促愈合药物。本文将蜂胶作为促愈合剂用于创伤模型，通过HE染色观察皮肤组织愈合过程，免疫组织化学染色动态观察血管内皮生长因子（vascular endothelial growth factor，VEGF）、血小板源性生长因子（platelet-deri