他克莫司处理的未成熟树突状细胞对小鼠炎症性肠病的作用及其机制研究.docVIP

他克莫司处理的未成熟树突状细胞对小鼠炎症性肠病的作用及其机制研究 　　[摘要] 目的 探讨他克莫司（Tac）处理的未成熟树突状细胞（imDC）对小鼠克罗恩病模型中炎性肠病的作用及其具体的机制。 方法 体外培养小鼠骨髓来源的树突状细胞（DC），加入Tac处理抑制其成熟，以2，4，6-三硝基苯磺酸（TNBS）灌肠法制作克罗恩病模型。将48只小鼠随机分为4组，分别为对照组、模型组、imDC组、Tac-imDC组，每组各12只。对照组给予50%乙醇溶液灌肠，其余三组分别给予TNBS灌肠造模，各组在灌肠前24 h和灌肠后24 h分别经尾静脉输注相应的PBS或者imDC，对照组和模型组经尾静脉输注PBS，imDC组输注1×106个未经Tac处理过的imDC，Tac-imDC组输注1×106个经过Tac处理过的imDC。每天记录小鼠一般情况，7 d后分别行疾病活动指数（DAI）、结肠大体形态损伤指数（CMDI）和结肠病理组织学（TDI）评分，取小鼠结肠组织行酶联免疫吸附试验（ELISA）检测白介素-10（IL-10）、转化生子因子-β（TGF-β）和白介素-17（IL-17）的水平变化。 结果 相比于对照组，模型组的DAI、CMDI和TDI评分明显升高[DAI：（0.75±0.10）比（5.12±1.05）分；CMDI：（0.70±0.12）比（6.52±1.18）分；TDI：（0.34±0.03）比（5.53±1.12）分；均P 中国论文网 /6/view-7218258.htm 　　[关键词] 他克莫司；树突状细胞；小鼠炎症性肠病；克罗恩病模型 　　[中图分类号] R574 [文献标识码] A [文章编号] 1673-7210（2016）01（a）-0025-06 　　Research of the function and mechanism of Tacrolimus-treated dendritic cells on mouse inflammatory bowel disease 　　WEI Danyun ZHANG Hong PEN Rui ZHANG Ying 　　Department of Pharmacy， Renmin Hospital of Wuhan University， Hubei Province， Wuhan 430060， China 　　[Abstract] Objective To investigate the effect of Tacrolimus-treated immture dendritic cells （imDC） on the Crohn’s mice models induced by 2， 4， 6 - trinitrobenzene sulfonic acid （TNBS ） and the action machnasim. Methods Dendritic cells were obtained from the murine bone marrow and treated with Tac to maintain its immature， and Crohn’s mice models were induced by TNBS. 48 mice were randomly divided into four groups： control group， model group， imDC group and Tac-imDC group， evey group contains 12 mice. The other three groups were iuduced to Crohn’s models by instillation of the 50% ethanol/TNBS enema. And the control group was instilled 50% ethanol enema. 24 hours before and after instill enema， PBS or cells was injected via tail vein. Control group and model group were injected PBS； the imDC group were injected DC that did not treated by Tac； the Tac-imDC group were injected imDC that treated by Tac. Then the general situation of mice were observed. After one week， the disease acti