胡黄连苷Ⅱ保护脑缺血损伤的最佳治疗剂量和时间窗.docVIP

胡黄连苷Ⅱ脑缺血损伤最佳治疗剂量及时间窗 Ⅱ可抑制脑缺血损伤大鼠缺血半影区神经细胞凋亡，缩小脑梗死体积，改善动物的神经行为和功能，但其最佳剂量及时间窗通过正交试验法验证胡黄连苷Ⅱ对大鼠脑缺血损伤的神经保护作用并优化其最佳治疗剂量及时间窗甲苯胺蓝染色显示2.0 h，胡黄连苷Ⅱ10 mg/kg；流式细胞术检测显示为1.5 h，胡黄连苷Ⅱ10 mg/kg；免疫组织化学染色法显示为2.0 h，胡黄连苷Ⅱ10 mg/kg；Western blot检测显示为2.0 h，胡黄连苷Ⅱ10 mg/kg；RT-PCR显示为1.5 h，胡黄连苷Ⅱ10 mg/kg。实验根据用药剂量最小化和治疗时间窗最大化的原则综合评价显示，胡黄连苷Ⅱ脑缺血损伤的最佳治疗时间窗为脑缺血1.5-2.0 h，腹腔注射胡黄连苷Ⅱ剂量为10 mg/kg。胡黄连苷ⅡArticle: Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury, by Guangyi Liu, Li Zhao, Tingting Wang, Meizeng Zhang, Haitao Pei (Institute of Cerebrovascular Diseases, Affliated Hospital of Qingdao University, Qingdao, Shandong Province, China) Liu GY, Zhao L, Wang TT, Zhang MZ, Pei HT. Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury. Neural Regen Res. 2014;9(15):1437-1445. 欲获更多资讯： Picroside II for cerebral ischemic injury: optimal therapeutic dose and time window A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. But there is no final conclusion in its therapeutic time window and dose for cerebral ischemia. Guangyi Liu and colleagues from Affiliated Hospital of Qingdao University in China validated the neuroprotective effects of picroside II and optimized its therapeutic time window and dose in a rat model of cerebral ischemia. They found that the optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. These findings, which have been in the Neural Regeneration Research (Vol. 9, No. 15, 2014), suggest t