基质细胞衍生因子1对内源性神经干细胞的趋化迁移作用.docVIP

基质细胞衍生因子1对内源性神经干细胞的趋化迁移作用 苏?稳，丁?鹏，王进昆，张?浩，牟临杰，王?波，刘景传，龚光辉，王崇谦 昆明医学院第一附属医院神经外科，云南省昆明市?650032 Stromal cell derived factor 1 effects on migration of endogenous neural stem cells Su Wen, Ding Peng, Wang Jin-kun, Zhang Hao, Mu Lin-jie, Wang Bo, Liu Jing-chuan, Gong Guang-hui, Wang Chong-qian Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China 摘要? 背景：目前研究表明，基质细胞衍生因子1在参与趋化迁移内源性神经干细胞中起着非常重要的作用，但其具体迁移机制尚不明确。 目的：观察外源性基质细胞衍生因子1 对大鼠内源性神经干细胞的趋化迁移作用及海马区神经干细胞的激活增殖情况。 方法：通过向SD大鼠海马区上大脑皮质内注射外源性基质细胞衍生因子1(注射量为5 μL，质量浓度为500 μg/L)建立动物模型，于3，7，14，21 d后灌注取脑，通过石蜡切片免疫组织化学检测大鼠皮质内注射区及海马区nestin阳性细胞表达情况，并设实验对照组与空白对照组。 结果与结论：石蜡切片免疫组织化学显示：实验组注射区周围及海马区nestin表达阳性细胞的数量随时间推移逐渐增多，3，7 d时注射区及海马区nestin表达阳性细胞少量表达，14 d时注射区及海马区nestin表达阳性细胞进一步增多，并向注射区形成明显的迁移趋势，21 d时注射区及海马区nestin表达阳性细胞更多。实验对照组及空白实验组未见上述表现。结果表明外源性基质细胞衍生因子1 可能诱导海马区神经干细胞的增殖分化，参与内源性神经干细胞的趋化迁移过程。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 全文链接： 关键词?：?干细胞；?神经干细胞；?干细胞因子及调控因子；?基质细胞衍生因子1；?内源性神经干细胞；?趋化迁移；?海马； ?? Abstract： BACKGROUND:Stromal cell derived factor 1 in chemotactic migration of endogenous neural stem cells plays a very important role, but the specific migration mechanism is unclear OBJECTIVE:?To observe the effects of exogenous stromal cell derived factor 1 on chemotactic migration and proliferation of neural stem cells in the rat hippocampus METHODS:Exogenous stromal cell derived factor 1 (5 μL, 500 μ/L) was injected into the hippocampus of Sprague-Dawley rats to establish animal models. Brain tissues were taken after days 3, 7, 14 and 21 of perfusion to prepare paraffin sections. Thereafter, nestin expression in the injection region and hippocampus was detected using immunohistochemical method. Experimental control and blank control groups were set. RESULTS AND CONCLUSION:?Paraffin section immunohistochemical results displayed the number of nestin-positive cells in the injection and the hippocampus was gradually increased. At 3