百合育种教案分析.ppt

基因重组是指生物体进行有性生殖的过程中，控制不同性状的基因的重新组合。 异源多倍体：生物学名词，指不同物种杂交产生的杂种后代经过染色体加倍形成的多倍体。 性状分离：指在后代中同时出现显现性状和隐性性状。 基因分离定律的实质:位于一对同源染色体上的等位基因,具有一定的独立性,生物体在进行减数分裂形成配子时,等位基因会随着同源染色体的分开而分离,分别进入到两个配子中,独立地随配子遗传给后代.? Two different types of polyploid progenies were analyzed by genomic in situ hybridization (GISH) in order to estimate the extent of homoeologous recombination。 ALA-triploids and AALA-tetraploids showed considerable intergenomic recombination explainable by homoeologous pairing in LA-hybrid during the origin of 2n-gametes。 INTRODUCTION The genus Lilium has been classified into seven sections with more than 80 species Plant height, disease resistance, and flower color and size.（价值） Interspecific hybridization is inevitable sterile due to disturbed chromosome pairing during meiosis 2n-gametes have been successfully used for producing sexual polyploids. An advantage of 2ngametes is that the F1 hybrids can directly be used for further crossing. When introgression of specific traits is the aim, the use of 2n-gametes and sexual polyploidization is preferable as compared to mitotic chromosome doubling We studied the extent of intergenomic recombination in two different types of polyploid progenies derived from two interspecific Lilium hybrids Diploid hybrids L. longiflorum ′ L. rubellum (LR) and L. longiflorum ′ Asiatic hybrid (LA). MATERIALS AND METHODS Plant materials Chromosome preparation DNA isolation and probe preparation In situ hybridization LL ×RR LL×AA ↓ ↓ LR(F1)× ‘Snow Queen’, LA (F1) ↓ LLR(2n=3x=36) LLRR ×OO LA×AA LA ×OO ↓ ↓ ↓ OLR ALA OLA diploid cultivars of Asiatic hybrids, viz., ‘Montreux’, ‘Puccini’ and ‘Meribel’, and accession number ‘78251’ were used as female parents grown in a greenhouse Day (17-20℃) Night (14-18 ℃) Chro