免疫共沉淀ChIP.docVIP

ChIP protocol Basic on EZ-ChIP? Chromatin Immunoprecipitation Kit Upstate Catalog # 17-371 A. In Vivo Crosslinking and Lysis 1、 Stimulate or treat 4.5 x 107 cells on a 10cm dish as appropriate. (25K、5K for 4h) 2、Cross link protein to DNA by adding formaldehyde directly to culture medium to a final concentration of 1% and incubate 10 minutes at room temperature. (add 270ul 37% formaldehyde into 10ml of growth medium on plate) 3. Add 1 ml of 10X glycine to each dish to quench unreacted formaldehyde. Swirl to mix and incubate at room temperature for 5 minutes. 4、Aspirate medium, wash cells twice