本实验主要研究普洱茶降血脂的功能并寻找其降血脂的有....docVIP

摘 要 本实验主要研究普洱茶降血脂的功能并寻找其降血脂的有效部位，为进一步分离提取有效成分提供实验依据。采用脂代谢紊乱模型——预防性给受试物法，对普洱茶（生茶）、普洱茶（熟茶）水提物的降血脂效果进行比较；对经贮藏的普洱茶（熟茶）降血脂效果进行了评价；然后选用系统溶媒（氯仿、乙酸乙酯、正丁醇）对普洱茶（熟茶）的水提物进行分层萃取，观察普洱茶（熟茶）各萃取层对高脂饮食大鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL-C）和低密度脂蛋白（LDL-C）的影响，从而明确普洱茶（熟茶）降血脂的有效活性部位。实验结果表明： 1、普洱茶（熟茶）有降低高脂饮食小鼠血脂水平的功能。普洱茶（生茶）具有降低高脂饮食小鼠血清甘油三酯（TG）的功能。 2、普洱茶（熟茶）不影响正常饮食小鼠的血脂水平. 3、从百分比角度分析普洱茶（熟茶）的降血脂效果可能具有一定的量效关系，总体表现是高剂量降血脂效果较好。普洱茶（生茶）对TG的量效关系而言，普洱茶（生茶）低剂量的效果好。从单因素方差的角度分析普洱茶（生茶）对于TG、LDL-C的下降有量效关系；而对于TC、HDL-C的量效关系不显著。普洱茶（熟茶）对于TG、TC、LDL-C、 HDL-C的量效关系不显著。 4、普洱茶（熟茶）高剂量组对动脉粥样硬化和冠心病有预防作用。普洱茶（熟茶）中剂量组对冠心病有预防作用。普洱茶（熟茶）超高剂量组对动脉粥样硬化有预防作用。普洱茶（生茶）对动脉粥样硬化和冠心病的预防作用不显著。 5、经贮藏的普洱茶（熟茶）具有降低高脂饮食小鼠血脂的功能。 6、乙酸乙酯层是普洱茶（熟茶）降血脂的有效部位。 7、氯仿萃取物、正丁醇萃取物、萃取剩余物对动脉粥样硬化和冠心病的预防作用不显著；乙酸乙酯萃取物对动脉粥样硬化和冠心病有预防作用。 关键词：普洱茶；降血脂；有效部位 Study on the effect of pu-erh tea on reducing blood lipids and its physiologically active part Zhao Tingting (Tea science) Directed by Zhou Binxing ABSTRACT This experiment mainly studies on the effect of pu-erh tea in reducing blood lipids and its physiologically active part. The experiment adopts the model of metabolic disturbance of lipid—preventive tested sample. Firstly, compare the effect of fermented pu-erh tea and non-fermented pu-erh tea in reducing blood lipids. Secondly, evulate the effect of different storage time pu-erh tea in reducing blood lipids. Then using systematic solvents (Chloroform，ethyl acetate，Butanol) extract the fermented pu’er teaObserve the effects of different extracts on reducing the serum levels of TC、TG、HDL-C、LDL-Chigh-fat-diet rat. The results are indicated as follows: 1、Fermented pu-erh tea can reduce blood lipids. Non-fermented pu-erh tea can reduce the serum of TG. 2、Fermented pu-erh tea doesn’t influence normal-diet mice’s serum of blood lipids. 3、From the percentage point to analyze: fermented pu-erh tea has the dose dependent relationship, and high dose of fermented pu-erh tea has the better effect; low dose of non-fermented pu-erh tea is effective to the serum of TG.. F