羟基喜树碱中华文章重点分析.docVIP

10-羟基喜树碱对CIA 的免疫调节作用 王燕1黄贺梅2 胡晓舟1 胡慧华1 1郑州大学第五附属医院风湿免疫科 450052 主任医师 Email:wangyanh_1963@163.com 2郑州铁路职业技术学院医学技术系 450052 通讯作者:胡慧华,Email:hhhu@ 尊敬的编辑部负责同志： 《10-羟基喜树碱对类风湿关节炎患者T淋巴细胞抑制作用研究》获得2011年河南省科技厅计划项目基金10万元， 为河南省重点科技攻关项目。属于省部级立项课题论文 [摘要] 目的 探讨10-羟基喜树碱对小鼠胶原诱导性关节炎（CIA）的免疫调节作用。方法 通过10-羟基喜树碱干预CIA 模型，观察小鼠发病情况，运用免疫组化技术观察局部关节病变程度；FACs 分析T 细胞亚群变化；ELISA 检测细胞培养上清中TGF-β、IFN-γ、IL-17 水平,检测Treg、Th1、Th17 细胞相关转录因子表达水平。结果 经过10-羟基喜树碱干预，小鼠自身反应性T细胞的增殖能力被显著抑制。与CIA组比较，10-羟基喜树碱组Treg 细胞百分比上升，Th1、Th17 细胞百分比明显降低；各细胞分泌细胞因子的能力也发生相应的变化，其中TGF-β 上升，IFN-γ、IL-17 降低；Treg 细胞转录因子Foxp3 基因水平提高。结论 10-羟基喜树碱可通过抑制CIA 小鼠自身反应性T细胞增殖，上调小鼠体内Treg 细胞并抑制Th1、Th17 细胞的表达而发挥免疫调节作用，有效降低小鼠发病严重度和关节炎症。 [关键词] 10-羟基喜树碱；CIA；Treg；Th1；Th17 Immunoregulatory roles of HCPT in collagen induced arthritis WANG Yan,HU Xiao zhou,HU Hui hua .The Fifth Affiliated Hospital of Zhengzhou University, Division of Rheumatology.450052,China.HUANG He mei.Zhengzhou Railway Vocational and Technical College of Medical Technology.450052,China.HUANG He mei, Corresponding author:HU Hui hua ,Email:hhhu@ [Abstract] The study aims to explore the immunoregulatory roles of HCPT on collagen-induced arthritis. DBA/1 mice were treated with HCPT, and then used to establish collagen induced arthritis (CIA).After disease onset, the incidence, severity, and joint histopathology were evaluated. We detected the percentage of Th subsets such as Treg, Th1 and Th17. ELISA assay was used to detect the levels of related cytokines in the culture supernatants. Mice in the HCPT -treated group showed alleviated disease symptoms, and less infiltration of inflammatory cells in the joint sections. The proliferation of CII-specific cells was inhibited in the HCPT treatment group. HCPT treatment also increased the percentage of Treg cells, but decreased the percentage of Th1 and Th17. The results of this study indicated that HCPT treatment can reduce the incidence and severity of CIA by up-regulating Treg and down-regulating Th1 and T