三种不同方法提取吴茱萸果实挥发油成分的GC-MS-最新重点分析.docVIP

三种不同方法提取吴茱萸果实挥发油成分的GC-MS分析 刘应蛟，徐贝，喻亚飞，龚力民，曾晓艳，刘塔斯* （湖南中医药大学药学院，湖南 长沙 410208） [摘要]：目的 用GC-MS法分析吴茱萸果实中挥发油的化学成分,为其不同方法提取提供实验依据。方法 采用水蒸气蒸馏法、索氏提取法和超声提取法对吴茱萸果实挥发油进行提取，用GC-MS法测定，结合NIST05数据库对分离的化合物进行鉴定，并采用归一法计算各组分的相对峰面积。结果 3种不同方法提取吴茱萸果实挥发油的化学成分有差异，分别分离鉴定出57、51和38个组分，共有成分25个，其中主要成分为β-月桂烯、β-罗勒烯、a-蒎烯、β-芳樟醇、[1S-(1α,2β,4β)]-1-乙烯基-1-甲基-2,4-二（1-甲基乙烯基）-环己烷、石竹烯、吉马烯、α-布藜烯和氧化石竹烯等。结论 水蒸气蒸馏法和索氏提取法在得到具有一定药理活性的石竹烯、a-蒎烯、榄香烯及β-罗勒烯等，能够达到同样的提取效果。因此可以综合考虑选择所需要的组分而选择合适的提取方法。 [关键词] 吴茱萸；水蒸气蒸馏法；索氏提取法；超声提取法；挥发油；GC-MS Three different exaction methods on the constituents of Volatile oil from Evodiae Fructus with GC-MS analysis LIU Yingjiao, XU Bei, YU Yafei, GONG Limin, ZENG Xiaoyan, LIU Tasi* (Pharnacy college, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) [Abstract] Objective: To analyze the chemical components of the essential oil of the Evodiae Fructus with GC-MS and provide experimental basis for its different exaction methods. Methods: The volatile oils of Evodiae Fructus were extracted by different methods such as steam distillation，Soxhlet extraction and ultrasonic. The chemical compounds were identified by GC-MS method and NIST05 database. The relative content of each component was calculated by area normalization．Results: There are some differences in chemical compounds of the essential oil of the Evodiae Fructus by 3 different methods. 57，51 and 38 violatile components were identified separately, and there were 25 same components. The main components are .beta.-Myrcene, .beta. -Ocimene, .alpha.-pinene, .beta.-Linalool, [1S-(1.alpha.,2.beta.,4.beta.)]-1-ethenyl-1-methyl-2,4-bis(1- methylethenyl)-Cyclohexane, Caryophyllene, Germacrene D, .alpha.-Bulnesene and Caryophyllene oxide etc.. Conclusion: Steam distillation and Soxhlet extraction meets the same part in extracting of chemical compounds with certain pharmacological activities, Such as Caryophyllene，.alpha.-pinene，Elemene and .beta. -Ocimene etc.. So we should be taken into consideration of components of the re