血凝的反应曲线精要.ppt

* * In this example, the Fib-C result is failed with a coag error of “Baseline SD exceeds error limit”. As with the PT-RP test, the SD of the first 1 second of Fib-C absorbance measurements is evaluated. The SD must be less than 2. If greater than 2, the test will fail. This error occurs because the sample is already clotting at the time the TOP starts collecting absorbance measurements. Remember the Fib-C assay is a rate reaction, which means the quicker the sample clots, the higher the fibrinogen. Therefore, the most appropriate way to handle this sample is to rerun it with the Fib-C H which is a 1:20 dilution. * * This is the reflexed Fib-C H. The fibrinogen obtained is 998 mg/dL. This result may be reported as it is within the linearity of the Fib-C H test. * 实验室除了PT\APTT\FIB-C实验外，还有很多其他的测试项目，有凝固法的测试，还有发色底物法，免疫比浊法。ACL TOP的曲线是根据检测到得吸光度而绘制成的，与反应类型无关。发色底物法和免疫比浊法有不同的运算法则来评估曲线。另外，报告的单位不是直接测量得到得，而是需要定标曲线来转换。 * ACL TOP上其他方法学的运算法则是： 终点减起点法 线性动力学 这些法则也是用来评估检测到得数据的。如果数据符合所有运算法则，就会通过定标曲线将数据转化成样本最终的结果，%或ng/ml等等。如果发生错误，也有可能与定标曲线有关。 * * This is an example of an acceptable D-Dimer reaction curve. The D-Dimer assay is an Immunologic assay in which Latex particles coated with antibody directed towards the DDimer domain are combined with a patient sample. Those particles bind to DDimer in the patient sample and agglutinate causing an increase in absorbance. The TOP monitors the change in absorbance. This change is directly proportional to the amount of DDimer present in the sample. The algorithm used for DDimer determination is final minus initial. This average of a range of points at the beginning of the curve is subtracted from an average of a range of points at the end of the curve. For this sample the difference between the 2 averages is 22 mAbs. * * The mAbs obtained is then compared to the calibration curve to determine how much DD in ng/mL is present in the sample. This is an example of a D-Dimer calibration curve. The curve is built by performing a DD on a ca