RNA干扰技术应用的论文.docVIP

1RNA干扰技术及其应用研究RNA Interference Technique and Its Application陈若飞; 畜牧与饲料科学 , Animal Husbandry and Feed Science, 2009年 05期RNA干涉作用,是生物界一种古老而且进化上高度保守的现象。是基因转录后沉默作用(PTGS)的重要机制之一。它能定向关闭生物体内某一基因,使其不发挥作用,同时不影响其他基因的功能,便于研究特定基因的功能。在后基因组时代的基因功能研究、基因治疗和药物开发中具有广阔的应用前景。RNA interference(RNAi) is an ancient and highly conservative phenomenon during biological evolution.It is also one of the important mechanisms of post-transcriptional gene silencing(PTGS).It can directionally close a gene and prevent its functions in vivo,not affecting the functions of other genes at the same time.This is propitious to study the functionds of particular genes.Therefore,RNAi has broad application prospects in gene function,gene therapy and drug development in the post-genome era. 2RNA干扰技术的应用现状及研究进展The Application and the Research Progress of RNA Interference Technique王春蕾; 赵博; 丛斌农业科技与装备 , Agricultural Science Technology and Equipment, 2009年 03期RNA干扰是指外源双链RNA进入细胞以后引起的与其同源mRNA特异性降解的现象,它参与真核生物抵抗病毒侵染,阻断转座子的异常活动,调控基因表达,并广泛存在于生物界。通过介绍RNA干扰技术的发现过程,探讨该技术的作用机理和研究进展,进而提出该技术的未来发展方向。RNA interference (RNAi) is all evolutionarily conserved eukaryotic adaptive response that leads to the specific degradation of target mRNA species in response to cellular exposure to homologous double-stranded RNA molecules. It is an endogenous pathogenic nucleic acids,and regulates the expression of protein-coding genes.This article deals with the discovery process of RNA interference technique and its action mechanism and research progress and finally points out its developing trend. 正文：RNA干扰(RNA interference,RNAi)现象是一种进化上保守的抵御转基因或外来病毒侵犯的防御机制。将与靶基因的转录产物mRNA存在同源互补序列的双链RNA(double strand RNA,dsRNA)导入细胞后,能特异性地降解该mRNA,从而产生相应的功能表型缺失,这一过程属于转录后基因沉默机制(posRNA干扰与神经退行性疾病的治疗?RNA interference and the therapy of neurodegenerative disease金岚芸; 林琳; 中国临床康复 , Chinese Journal of Clinical Rehabilitation, 2006年 38期??上海交通大学基础医学院神经生物实验室; 上海交通大学基础医学院神经生物实验室 上海市; 目的:神经退行性病变是人类神经系统的严重疾病之一,对于神经退行性疾病的治疗目前尚无有效方法,本文探讨RNA干扰技术运用于该病动物模型中的有效性,从而为进一步运用于临床提供可能。资料来源:应用计