2.2氯霉素对V.fischeri的Hormesis效应-同济大学学报.docVIP

氯霉素对费氏弧菌024 h的及其机制初探 于岩， 游瑞容1, 2，2，林志芬2 (1．福州大学 材料科学与工程学院，福建350108；2． 摘要: 为了探究抗生素对发光菌的Hormesis效应，选择费氏弧菌（Vibrio fischeri, V.fischeri）为受试生物，氯霉素为研究对象，测定了0~24 h下氯霉素对V.fischeri的发光强度（）及生长量（OD600）的作用，探讨氯霉素对V.fischeri的及其可能机制。结果表明，氯霉素对V.fischeri的发光强度具有明显的促进作用，且仅出现在细菌生长的延滞期。根据V.fischeri的发光机制，结合Gaussian量子化学计算结果，即氯霉素的最正氢电荷为0.244，远大于烟酰胺腺嘌呤二核苷酸（NADH）的最正氢电荷0.135，提出氯霉素通过提供质子促进荧光反应，进而产生Hormesis效应的可能机制。 关键：HormesisVibrio fischeri；氯霉素暴露时间 Chloramphenicol Hormesis effects about time-dependent and mechanism to Vibrio fischeri YU Yan1, YOU Ruirong1,2, YAO Zhifeng2, LIN Zhifen2 (1. College of materials science and Engineering, Fuzhou University, Fujian province 350108, China; 2. State Key Laboratory of pollution control and resource research, Tongji University, Shanghai 200092, China) Abstract：In order to reveal the Hormesis effect of antibiotics on bacterial, the adverse effects of chloramphenicol on the luminescence and cell proliferation (0~24 h) of Vibrio fischeri (V.fischeri) were investigated. It was found that an obvious stimulation effect at the low concentration would occur on the luminescence intensity at the lag phase of V.fischeri under the action of chloramphenicol. A possible mechanism hypothesis of the stimulation effect was then proposed, based on the light emitting mechanisms and the quantum chemical parameters calculated by Gaussian 09. It was supposed that because of a higher value of the most positive charge of the H atoms of chloramphenicol than NADH, chloramphenicol can act as proton donors to provide protons which are essential for the light emitting process, instead of NADH, and stimulates the light emitting of the bacteria. Keywords：Hormesis; Vibrio fishcheri; Chloramphenicol; time-dependent Hormesis效应，即低剂量刺激效应是种区别于传统毒理学剂量-效应关系的特殊现象[1]，Southam和Ehrlich[2]在1943年研究红雪松提取物对真菌的作用时发现这一现象，并将其定名为Hormesis现象。此后，越来越多与Hormesis效应相关的文章被报道，然而，研究人员对Hormesis效应的研究主要仍集中在对其现象的发现上，对其发生的研究尚且不足，因此，对Hormesis效应的低浓度促进作用及其机制的研究显得尤为重要。 研究表明，Hormesis的产生和刺激程度除了受剂量的影响以外，还与化合物在生物体中的暴露时