生物分离工程-双语版 1.pptVIP

Bioseparation of proteins 曹学君 Cao Xuejun 联系方式： caoxj@ecust.edu.cn Chapter 1 Introduction 1 Introduction Biomaterial solution properties: (1) Dilute solution (2) Components complicate (3) Unstable (4) Batches difference Bioseparation requirements (1) High purity (2) Large scale (3) Economical (4) Biocompatible New words and vocabulary 1 cell fusion 2 interest protein 3 biocompatible 4 purification 5 biological material 6 plant cell culture 7 fermentation 8 strategies II The Need for bioseparation Process requirements (1) Safety (2) Quality (3) Amount (4) Preventing Posttranslational change (5) Low cost What will bioseparation do? (1) Modification of existing procedures (2) Developing new and novel bioseparation technology Difficulty of generalized algorithm processes and subtle difference of different proteins New words and vocabulary Protocol (方案） Algorithm ( 法则） Generalized (通用的，一般化的） Invaluable (有价值的） Inactivate (失活的） Therapeutic (治疗的) Viable (可行的） III Classification of bioseparation steps(1) Bioseparation procedures (1) Removal of insolubles (2) Isolation of product (3) Purification (4) Polishing III Classification of bioseparation steps (2) Bioseparation unit operation (1) Cell disruption (2) Centrifugation (3) Chromatography (4) Drying (5) Evaporation (6) Extraction (7) Filtration (8) Membrane separation (9) Precipitation III Classification of bioseparation steps (3) Quality requirements: Products free from: (1) detergent (2) endotoxins (3) proteases (4) immunogenic substance (5) microbiological contamination (6) modified proteins (7) nucleid acids (8) oligonucleotide (9) enterotoxins（肠毒素） III Classification of bioseparation steps (4) Therapeutic usage requirements: (1) Potency (2) Identity (3) Abnormal toxicity (4) Nucleic acids (5) Homogeneity (6) Absence of conflicting activity Process requirements: (1) Maximize product yield in i