患腹水综合症肉鸡的肺动脉转录组学精讲.doc

JIANGXI AGRICULTURAL UNIVERSITY 本 科 毕 业 论 文（设 计） 题目： 患腹水综合症肉鸡的肺动脉转录组学研究 学 院： 动物科学技术学院 姓 名： 学 号： 专 业： 动物医学 年 级： 二0 15 年 5 月  摘要 为了探讨肉鸡腹水综合症与肺动脉变化关系，从转录组生物信息的水平上对肉鸡肺动脉进行分析。本实验选取了75羽15日龄健康AA肉鸡随机分成对照组（20羽）和造病组（55羽），造病组（D）饲喂含0.3% Nacl的高盐水，对照组（N）饮正常水。在饲养过程中肉鸡出现明显腹水综合临床症状后颈静脉放血致死，并取RV/TV0.29的鸡肺动脉于液氮速冻，再放置-80℃保存，备用于转录组测序技术（RNA-seq）检测。结果表明：（1）腹水鸡和正常鸡相比，发现了895个差异表达基因。（2）根据GO富集分析，895个差异表达基因主要参与Ribosome，Translation，Chemokine and cytokine activityImmune system等多个。本实验揭示了患腹水综合症肉鸡的肺动脉基因转录现出了差异因此，为肉鸡腹水综合的发病机制提供了更新的信息，也研发肉鸡腹水综合新的药物靶点筛选提供一定依据和临床价值。 关键词：肉鸡；肉鸡腹水综合；RNA-seq Abstract An experiment was carried out to investigate the pulmonary artery changes of broilers with Ascites Syndrome on transcriptome biological level. 75 15-days Arbor Acre commercial broilers were randomly divided into control group (20) and disease group (55), and the control group broilers (N) was raised with tap water while disease group broilers(D) was given 0.3% Nacl water. During the trail, birds were killed when they showed obvious clinical symptoms, and the pulmonary arterys were collected and stored in -80℃ for RNA-seq test if the RV:TV of the bird was over 0.29 . Results presented that: compared to control group, (1) the disease groups have 895 differential expressed genes. (2)According to GO analysis, 895 differential expressed genes enriched to these biological progress such as Ribosome, Translation, Cytokine?cytokine etcr multiple metabolic pathways and cause them to occur biological changes. The experiment demonstrated that gene transcript level of pulmonary artery of the broiler with ascites syndrome had a remarkable difference compared to the normal broiler. Therefore, our results provided updated information for the pathogenesis research of broiler ascites syndrome, and also offered evi