长期施肥对土壤产甲烷与氨氧化微生物的影响.pdf

摘要 rRNA 肥土壤产甲烷速率接近为零。添加硝酸盐培养显著降低了土壤产甲烷古菌16S 基因拷贝数(pO．05)。 (Ammoniaarchaea)和AOB(Ammoniabacteria)的丰度和组成 oxidizing oxidizing 进行了分析。选取江西鹰潭红壤站长期定位施肥玉米地为试验地，该试验地设置有不 时施氮磷钾肥(NPK+OM)等施肥处理。结果显示，长期施用有机肥后，土壤中古 菌和细菌amoA基因的丰度均显著增加。施用有机肥的土壤中AOA的香农指数和丰 富度指数最高，施无机肥其次，不施肥对照最低。而AOB仅丰富度指数是施有机肥 处理高于不施肥对照。可见，相比于AOB群落结构，施肥对AOA群落结构影响更 大。系统发育分析显示不同施肥处理中，大部分AOA的序列属于1．1bthaumarchaea， thaumarchaea。 仅有1条序列属于1．1a—associated 关键词：红壤；甲烷产生速率；微生物多样性；产甲烷古菌；氨氧化古菌；氨氧化细 菌；DGGE；qPCR；PLFA Abstract Abstract aresensitiveindicatorsoftheclimateandsoil Soil conditions，and microorganisms the ofsoil havea onsoil may significantimpact change quality of fertilizerandmanureareanessential ofsoil applicationsinorganic component used to inarable amendmentsare productionsystems．These primarily managementcrop increasenutrient to canalsoaffectsoil availabilityplants，butthey about1．13millionkm2or1 1．8％ofthe landsurface，and soilscover country’S support 22．5％ofthenation’S soilsare and soils，and heavilyweatheringleaching population．Red anddeficienciesinavailable is to arecharacterizedlow nutrients，which by pH verylikely affect is instructiveto the effectsof