纤维连结蛋白在肾间质纤维化大鼠肾组织中的表达.doc

纤维连结蛋白在肾间质纤维化大鼠肾组织中的表达及意义 程红新1 杨晓萍1 蒋雅红3 林智峰1赵瑾2 陶林2 新疆石河子大学医学院，1第一附属医院肾病科，2病理科，3第三附属医院医务部，新疆维吾尔自治区石河子市 832008 摘要： 背景：纤维连结蛋白 Fibronectin，Fn肾间质纤维化（ RIF）β1（TGF-β1）的表达。 结果与结论：与正常组及对照组比较，梗阻时间越长，肾小管间质损害程度越重，纤维化越明显。造模后1 d大鼠肾脏组织FN、MMP-9表达水平即出现上调，造模后3d，TGF-β1表达水平出现上调，并均于第14天达最高水平，与同期正常组、对照组相比，差异有显著性意义（P 0.05）单侧输尿管梗阻β1；肾间质纤维化 The expression and its siginificance of Fibronectin in Rats at kidney with Renal Interstitial Fibrosis CHENG Hong-xin ,YANG Xiao-ping, ZHAO Jin，TAO Lin Department of Nephrology , the First Affiliated Hospital of Shihezi Medical University, Xinjiang 832000, China Abstract BACKGROUND: The increasing of the expression of Fibronectin played the important role in the process of the renal interstitial fibrosis . OBJECTIVE: To investigate the expression and the effects of fibronectin FN in Rats at kidney with renal interstitial fibrosis RIF . METHODS: seventy-two male Sprague-Dawley SD rats were randomly divided into normal control group （n 24） 、sham-operation group n 24 and UUO model group model group，n 24 by touching ball method．Rats in the model group were prepared for UUO model , the sham-operation group were only prepared for sham-operatio,and the normal chtrol group were prepared for nothing. Rats in every group were sacrificed 1，3，7 and 14 d after operation after model establishment for model group n 6 for each time ， HE staining，Masson staining，and immunohistochemical staining were used to evaluate the degree of renal tubulointerstitial fibrosis obstructed side for model group and detect the expression of FN 、MMP-9 and TGF-β1 in renal tissues．RESULTS AND CONCLUSION: Histological observations of renal tissues revealed that the degree of renal tubulointerstitial fibrosis of rats in model group was significantly increased as compared with the normal group and sham-operated group after UUO ,and it was increased gradually accompanying with the increasing of degree of obstruction. It was indicated by im