SCFcKit信号对膀胱癌T24细胞侵袭迁移影响及其相关机制研究.pdf

1 4 1. 7 2. 9 3. 16 4. 29 5. 32 33 38 A 39 B 41 C 44 D 45 SCF/c-Kit T24 1 c-Kit SCF SCF/c-Kit β- β-cateninβ-catenin β-catenin SCF/c-Kit β-catenin SCF/c-Kit T24 1. Western blot FCMT24 c-Kit 2. T24 SCF1 ng/mL24 h 3. SCF1 ng/mL24 hc-kit Imatinib100 nMPI3K-Akt Wortmannin1 μMMAPK MEK U012610 μMSTAT3 JSI-1245 μM β-catenin XAV-939 10 nM40minSCF 1 ng/mL 24h T24 Transwell T24 1Νο. 1308085ΜΗ114 BYKL 1203 1 4. Western blot T24 SCF 1 ng/mLPI3K-Akt Wortmannin1μM40 minSCF1 ng/mL Akt 5. T24 SCF 1ng/mL β-catenin 6. Western blot T24 GSK-3βStaurosporine 5 nM LiCl 10 mM 24 h β-catenin T24 GSK-3ββ-catenin 1. T24 c-Kit 12 %T24 c-Kit 2. T24 SCF1 ng/mL24 h 75.42 %38.99 %P 0.01 3. Transwell OD 26.25±0.960.096±0.004SCF OD 47.25±1.71 0.119±0.006P 0.01Imatinib100 nM+SCF1 ng/mL OD 26.75±0.960.091±0.012Wortmannin1μM+SCF1 ng/mL 26±2.160.096±0.004XAV-939 10 nM+SCF 1 ng/mL OD 26±0.820.103±0.002SCF P 0.01U012610 μM+SCF1 ng/mLOD 46.75±2.63 0.115±0.002JSI-1245μM+SCF1 ng/mLOD 47.75±2.99 0.114±0.004SCF P 0.05 4. T24 SCF 1 ng/mLAkt P 0.01 Wortmannin1 μM40 min SCF 1 ng/mL T24 Akt SCF P 0.01 5. T24 SCF1 ng/mLβ-catenin SCF 0 h T24 SCF 30 min 2 SCF 1 h SCF β-catenin 6. GSK-3βStaurosporine 5 nM LiCl 10 mM T24 β-catenin P 0.01 1. SCF/c-Kit PI3K-Akt T24 2. SCF β-catenin T24 3. SCF PI3K-Akt T24 GSK-3β 4. T24 GSK-3ββ-catenin SCF/c-Kit PI3K-Akt-GSK-3β-β-catenin T24 SCF/c-Kit T24 PI3K-Akt β-catenin -3β 3 Effect of SCF/c-Kit signaling on invasion and migration of T24 bladder cancer cells and its related mechanism1 Abstract Background: Bladder cancer is the most common malignant tumor of the urinary system and its high transfer rate and high recurrence rate has been the focus of study. C-kit receptor belongs to tyrosine kinases receptor family and it is expressed on surface of many cells. C-kit can regulate cell proliferation, survival, differentiation and migration when it is crosslinked by its lig