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Analysis and Isolation of Adipocytes by Flow Cytometry
Yang LI
2016-04-05
Majka SM, Miller HL, Helm KM, et al. Analysis and Isolation of Adipocytes by Flow Cytometry. Methods in enzymology. 2014;537:281-296. doi:10.1016/B978-0-12-411619-1.00015-X.
2
CONTENTS
Background
Aim of the research
Difficulty
Solution
Background
What are adipocytes (fat cells)?Major cells in the adipose tissue.
3
Background
Two kinds of adipocytes in human
4
Prospects of adipocyte biology
Background
Lipid store
Inhibit eating
Increase metabolism
Leptin
5
Prospects of adipocyte biology
Background
Adipokines
Involved in energy metabolism
Lipid store
6
Prospects of adipocyte biologySpecifically from adipose tissue
Background
Lipid store
Adipokines
Energy metabolism
7
Prospects of adipocyte biologyAdipokines play an significant role in biological function.
Background
8
Background
Flow Cytometry (FCM)
Principle diagram
9
Background
Volume,
Morphological complexity of cells,
Total DNA/RNA content,
Apoptosis,
Cell viability,
... ...
... ...
10
Aim of the research
A method for analysis and isolation free-floating adipocytes by Flow Cytometry.
Analysis
Flow cytometry (FCM)
Confocal laser scanning microscope (CLSM)
Isolation
Fluorescence-activated cell sorting (FACS)
11
Aim of the research
Analysis
Flow cytometry (FCM)
Confocal laser scanning microscope (CLSM)
Beckman Coulter--MoFlo? XDP
Analysis rate:100,000 events/sec.
Sort rate: 70,000 events/sec.
12
A method for analysis and isolation free-floating adipocytes by Flow Cytometry.
Aim of the research
Analysis
Flow cytometry (FCM)
Confocal laser scanning microscope (CLSM)
Quantitive
High resolution imaging
13
A method for analysis and isolation free-floating adipocytes by Flow Cytometry.
Aim of the research
Analysis
Flow cytometry (FCM)
Confocal laser scanning microscope (CLSM)
Imaging Flow Cytometer(Amnis)
12 Images for every cell
Quantitive
High resolution imaging
14
A method for analysis and
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