汪世华分子生物学7解读.pptVIP

Arabinose binds to AraC, changing its shape so it prefers to bind as a dimer to l1 and l2 and not to O2. This opens up the promoter to binding by RNA polymerase. If glucose is absent, the CAP–cAMP complex is in high enough concentration to occupy the CAP-binding site, which stimulates polymerase binding to the promoter. Now active transcription can occur. Autoregulation of araC AraC controls its own synthesis by binding to araO1 and preventing leftward transcription of the araC gene. 7.2.1 SD sequence 7.2.2 mRNA stability 7.2.3 rare codons 7.2.4 anti-sense RNA 7.2.5 CRISPR 7.2.6 overlappi