IDO对胃癌细胞移植瘤中Ecadherin、Ncadherin及IL6水平影响.pdfVIP

目 录 摘要．??????????????．????．．I ABSTRACT???????．．．??????．????工I 中英文对照表????????????????．．III 前言???????????????????．1日U舌????????? 材料和方法??????????????????2 结果???????????????????．7 讨论???????????????????10 结论???????????????????13 参考文献??????????????????．14 综述???????????????????．16 个人简介??????????????????．24 致谢???????????????????25 万方数据 山西医科大学硕士学位论文 l Do对胃癌细胞移植瘤中E-cadher i n、N-cadher i n及I L一6 水平的影响 摘要 目的检测转染吲哚胺2，3一双加氧酶(IDO)的MFC胃癌细胞移植瘤中E—cadhefin、 N．cadhefin及IL．6的表达水平，研究三者表达水平内在的相关性及与IDO内在的关联性。 方法①用我院消化科课题组经PCR和Western blm法检测证实已经成功转染脂质体IDO 的MFC胃癌细胞、转染pcDNA3．1的MFC胃癌细胞、MFC胃癌细胞创建615小鼠皮下 移植瘤模型，分为pcDNA3．1．IDO．MFC、pcDNA3．1．MFC细胞组细胞组、MFC胃癌细 胞组，观察各组小鼠的成瘤情况，待造模成功后，处死小鼠，处理皮下移植瘤块。②用 免疫组化方法(IHC)检测3组的E．cadherin和N．cadhefin表达情况，并分析每组E—cadllefin 和N．cadllefin的表达的差别及三组表达的差别。③用酶联免疫吸附试验(ELISA)检测3组 血清液IL一6的水平。 结果①在pcDNA3．1．IDO．MFC组，E．cadherin的表达明显减少，与对照组比较差异有统 计学意义(P0．05)，N．cadllefin的表达明显增多，与对照组比较差异有统计学意义(P 0．05)。@pcDNA3．1一IDO．MFC细胞组的血清液的IL一6水平较其他两组明显增高，且与 对照组比较差异有统计学意义(P0．05)。 结论pcDNA3．1．IDO．MFC细胞组中E．cadhefin表达下降，N．cadhefin表达上升，IL一6表 达升高，推测可能与IDO通过多种机制上调IL．6等细胞因子进而导致了上皮间质转换过 程有关。 关键词E．cadhefin；N．cadherin；IL一6；吲哚胺2，3一双加氧酶：上皮一间质转换 万方数据 山西医科大学硕士学位论文 Effects of IDO on level of E．cadherin、N．cadherin and IL．6 in gastric cancer bearing mice Abstract Obj ective：To detect the expression of E--cadherin and N··cadlaerin and the level of lL—-6 in MFC gastric cancer bearing mice，and f-uther to study their inherent relevance in gastric cancer cells xenograft model and inherent correlation、析th IDO． Methods：@Establish the model ofMFC gastri cancer bearing mice，they were divided into three groups，MFC gastric cancer group，pcDNA3．1 transfected group，pcDNA3．1-IDO transfected group．②Use the immuohistochemistry to detect the expression of E-cadherin and N-cadherin in different groups．@ELISA was used for detection of the expression level of serum IL-6 in these three groups． Results：①In pcDNA3．1-IDO transfected group，the expression of E-cadherin protein Was reduced clearly compared嘶也the other groups，the differences was