Repairmechanisms.pptVIP

Repair mechanisms 1. Reversal of damage 2. Excision repair 3. Mismatch repair 4. Recombination repair 5. Error-prone repair 6. Restriction-modification systems 1. Reversal of damage Enzymatically un-do the damage a) Photoreactivation b) Removal of methyl groups Photolyase breaks apart pyrimidine dimers 1a. Photoreactivation Photolyase: binds a pyrimidine dimers and catalyzes a photochemical reaction Breaks the cyclobutane ring and reforms two adjacent T’s 2 subunits, encoded by phrA and phrB. Conversion of 6-O-methyl-dG back to dG 1b. Removal of methyl groups 6-O-methylguanine methyltransferas