细菌培养实验讲义.pptVIP

Medical Microbiology Experiment (2) routine cultivation of bacteria Experimental Contents 1. Media for routine cultivation of bacteria 2. Techniques for bacterial cultivation and inoculation--bacteria isolation cultivation inoculation 3. Growth characteristics of bacteria on/in different media 4. Metabolic activities of bacteria 5. The physical effect on bacteria and commonly used sterilizers--UV sterilization 1. Media for routine cultivation of bacteria Media--- The basic ingredients of media include carbon source、nitrogen source、 inorganic salt 、growth factor and distilled water etc. Classification of culture media According to function, in general, media can be classified five types: 1. basal medium (Agar plate medium) 2. nutrient medium (Blood agar plate medium) 3. selective medium (SS medium ) 4. differential medium (EMB medium) 5. anaerobic medium (Beef medium) According to physical properties, media can be classified three types: 1. solid medium ( 1.5～2.5% of agar): agar plate /slant medium 2. semi-solid medium (0.3～0.5% of agar) 3. liquid medium 2. Techniques for bacterial inoculation Bacterial inoculation is a basic technique of microbiology. The operation must be under sterile condition. 1) isolation cultivation 2) pure cultivation 1) Isolation cultivation streak a bacterial culture on a nutrient agar plate to separate the individual bacterial cells, each isolated cell will develop into a pure colony after incubation. continuous streak method quadrant streak dilution method 2) Pure cultivation transfer a single colony from agar plate or a pure slant culture to liquid medium, slant or semisolid medium to get a pure culture. 3. Phenomena of bacterial growth on agar plate (on blood agar plate) in l