利多卡因在关节软骨培养模型中对软骨细胞活性影响.pdfVIP

Abs仃act ABSTRACT Objective Lidocaine is a local anesthetic commonly used for treatment of osteoarthritis and perioperation analgesia．Cytotoxic effects of lidocaine on articular cartilage have been reported in the state of cell culture in vitro．This study evaluates the effects of lidocaine on cell viability using articular cartilage organ culture model that provides a physiological environment closer to one found in vivo． Methods Articular Cartilage of human female patients was lsolated from residual normal parts of femoral condyle operated by knee arthroplasty for osteoarthritis． Fresh cartilages with full thickness(diameter 4mm)were harvested by round drill． Articular cartilage was cultured in the media for one week，the integrity of organ culture model was tested．Organ culture of articular cartilage was exposed to clinically relevant concentrations(0％，1％，2％)and different exposure durations(30， 60，120 minutes)of O．9％saline and lidocaine．The cytotoxic effects on articular cartilage were evaluated by the changes of histological feature and cell viability which determined quantificationally by histological HE staining and MTT assay． Results Fresh articular cartilage showed little changes in histologic struture and cell viability when cultured in media fn vitro for up to one week．Compared to 90％cell viability in 0．9％saline，cell viability was decreased to 79．1±1．5％，69．8±3．1％and 56．0±1 O．4％respectively when articular cartilage was cultured in 1％lidocaine for 30mins．60mins and 1 20mins．Expose to 1％and 2％lidocaine for one hour resulted in about 77．9±4％and 69．9±2．7％cell viability While 0．9％saline exposure over the same amount of time kept 9 1．8±1．7％cell viability． Conclusions human articular cartilage maintained the integrity of histology and cell viability when cultured in vivo for up to one week．Exposure of this biologically and structurally intact articular cartilage，which closely approximates the physiological environment in vivo，to lidocaine decreases cell viability in