巢湖蓝藻藻蓝蛋白提取纯化和微囊藻毒素LR去除研究.pdfVIP

巢湖蓝藻藻蓝蛋白提取纯化及微囊藻毒素．LR去除研究 炭纤维用量为109／L，温度为35。(2，pH值为8．0，MC．LR去除率最高达到92．9％。 固定化藻毒素降解菌对pH值，温度具有一定的耐受性，能够在pH5～pH9、 10。C～35。C范围内有效地去除MC．LR。 关键词：蓝藻，藻蓝蛋白，微囊藻毒素一LR(MC．LR)，活性炭纤维 Abstract Abstract Recently,Chaohu cyanobacteria bloom outbreaks frequently,cyanobacteria salvage has become an important emergency measures for control of cyanobacteria， but salvaged cyanobacteria improper disposal Can cause secondary pollution．This study aimed at development and technical study of Chaohu cyanobacteria high value—added products，and focused on C—phycocyanin extraction and purification research，and using immobilized microbial technology to remove generated microcystin-LR Was studied． In this study,the process of’freeze-thaw broken—salting-out-aqueous two—phase extraction—ultrafiltration”Was used to extract andpudfy C-phycocyanin， effects of freeze—thaw times，thawing temperature and the ratio of cyanobacteria to water(the concentration of cyanobacteria)on purity and yield of C—phycocyanin were studied；effects of ammonium sulfate saturation and salting—out steps on purity and yield of C-phycocyanin were studied；effects of aqueous two—phase extraction system on purity of C-phycocyanin were studied，such as molecular weight and the concentration of polyethylene glycol，the inorganic salts，the concentration of potassium phosphate and pH value；the effect of molecular weight of ultrafiltration Was studied．The results showed that；the optimum conditions of freeze-thaw are using flesh cyanobacteria as the raw material，the freezing temperature-20。C， thawing temperature 20。C，freeze-thaw twice，the ratio of cyanobacteria to water(the concentration of cyanobacteria)1．25％：the optimum conditions of salting—out are two。step salting-out．20％and 35％ammonium sulfate saturation；the optimum conditions of aqueous two-phase ale pH 6．0，the concentration of PEG4000 1 6％， the concentration of KHP 1 2％：the optimum condition of ultrafiltration is 3 0KDa ultrafiltration．Finally the purity of C-phycocyanin up to 4．8，and