究极版.docVIP

Chem ical Composition and Antioxidant Components of EssentialO ils from Leaves, Bark and Root of M yrica rubra ZHONGRui2min1,2,ZHANGZhen2ming2,WANGYu2mei2,ZENGQing2xiao1,PENGHua2gui3 ( 1. College of L ight Industry and Food Science, South China University of Technology, Guangzhou 510640, China;2. Yingdong Co llege of B iotechnology, Shaoguan Co llege, Shaoguan 512005, China; 3. D adingshan MountainA dm inistration of N anling N ational N ature Reserve, Shaoguan 512726, China) Abstract: Essential oils from leaves, bark and root of Chinese waxm yrtle (M y rica rubra var. astropu rea Tsen) , grow ing w ild inN anling N ational N ature Reserve, have been analyzed fo r their chem ical compo sition and investigated for their antioxidant compo2nents. TheGC2MSanalysisaswellascomparisonoftheKovatsretentionindices(KI)ofelutionpeakswithliteraturedatawereused for compound identification of the essential oils; TLC2screeningmethodwasusedforanalysisofantioxidantcomponentsinthe o ils. The essential o ils ofM. rubra bark and roo t are both dom inated w ith 52hydroxycalamenene(74.66%and60.32%)ando leic acid ( 7. 74% and 11. 39% ),respectively, whereas,he leaves o il is constituted mainly by 52hydroxycalamenene( 30. 44% ), caryophyllene ( 11. 61% ), isocaryophyllene ( 10. 17% ), ( E )2nerolidol(9.57%)andoleicacid(4.40%),respectively. The composition m atrixes of the essential oils of M.rubraleaves, bark and root exp ress the differential comp lexityof secondary m etabolites in this species. 52Hydroxycalameneneisidentifiedastheprincipalantioxidantinallthreeessentialoils.However, the sesquiterpenes in leaves o il also show po sitive 2, 22diphenyl212picrylhydrazyl(DPPH)radicalscavengingactivityincertain extent.Key words: M y rica rubra var. astropu rea Tsen; essential oil; Kovats retention index; antioxidant activity 萜烯类(terpenes)物质是一类具有较强香气和生理活性的天然烃类化合物，在自然界广泛分布。萜类化合物普遍存在于植物体中，具有特殊功效的生理活性。对它们的分离、结构测定、化学合成及其应用研究是多年来的热点课题。 萜烯类物质，尤其是挥发油，通常都具有辛辣灼烧的感觉，有些有苦味。由于该类物质有很强的杀菌、消炎、止痛、抗癌等作用，它们与黄酮类物