Sepharose 4 Fast Flow matrix.docVIP

Sepharose 4 Fast Flow matrix Sepharose 4 Fast Flow is a highly cross-linked agarose matrix. In its preactivated CNBr form, it offers much improved performance when compared with the well established CNBr-activated Sepharose 4B. The Sepharose 4 Fast Flow matrix has higher rigidity and can thus be run at high flow rates (see Table 1). The higher mechanical strength of the cross-linked matrix makes it wellsuited for use in large columns. Scaling up a purification developed on CNBractivated Sepharose 4 Fast Flow is therefore simple and more predictable. The coupled product is stable at low pH, whi